Parathyroid hormone receptors in avian bone cells [Chickens]

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 79; no. 6; pp. 2061 - 2063
• Main Authors: Pliam, Nathan B., Nyiredy, Katalin O., Arnaud, Claude D.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.01.1982; National Acad Sciences
• Subjects: Adenylyl Cyclases - metabolism; Animals; Binding sites; Binding, Competitive; Bone and Bones - metabolism; Bones; Cell culture techniques; Cell membranes; Cells, Cultured; Chick Embryo; Chickens; Cultured cells; Hormones; Kinetics; Minerals; Parathyroid Hormone - metabolism; Receptors; Receptors, Cell Surface - metabolism; Receptors, Parathyroid Hormone; Ungulates
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.79.6.2061

We have demonstrated binding of synthetic bovine parathyroid hormone (1-34) [bPTH-(1-34)] to embryonic avian bone cells in monolayer culture. The binding sites have qualitative and quantitative characteristics of a physiologically important parathyroid hormone (PTH) receptor. At apparent steady state (60 min at 24 degrees C), 5-10% of electrolytically labeled, receptor-purified125I-labeled bPTH-(1-34) bound specifically to the cells whereas nonspecific binding was less than 1% of the added labeled hormone. Scatchard analysis showed a single order of PTH binding sites (Kd=0.6 nM) with approximately 10,000 sites per cell. In this bone cell system, PTH bound to its binding site and stimulated cAMP accumulation over the same concentration range. Bovine PTH-(1-84) bound to the cells with the same apparent affinity as bPTH-(1-34).