Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota

• Published in: Science (American Association for the Advancement of Science) Vol. 338; no. 6103; pp. 120 - 123
• Main Authors: Arthur, Janelle C., Perez-Chanona, Ernesto, Mühlbauer, Marcus, Tomkovich, Sarah, Uronis, Joshua M., Fan, Ting-Jia, Campbell, Barry J., Abujamel, Turki, Dogan, Belgin, Rogers, Arlin B., Rhodes, Jonathan M., Stintzi, Alain, Simpson, Kenneth W., Hansen, Jonathan J., Keku, Temitope O., Fodor, Anthony A., Jobin, Christian
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 05.10.2012; The American Association for the Advancement of Science
• Subjects: Animal tumors. Experimental tumors; Animals; Azoxymethane - toxicity; Bacteria; Biological and medical sciences; Cancer; Carcinogens - toxicity; Carcinoma - chemically induced; Carcinoma - microbiology; Carcinoma - pathology; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - pathology; Colitis; Colitis - complications; Colitis - genetics; Colorectal cancer; Colorectal Neoplasms - chemically induced; Colorectal Neoplasms - microbiology; Colorectal Neoplasms - pathology; DNA Damage; E coli; Epithelial cells; Escherichia coli; Escherichia coli - genetics; Escherichia coli - pathogenicity; Experimental digestive system and abdominal tumors; Inflammation; Interleukin-10 - genetics; Intestines - microbiology; Intestines - pathology; Medical sciences; Metagenome - genetics; Metagenome - physiology; Mice; Mice, Mutant Strains; Microbiology; Microbiota; Microorganisms; Online; Patients; Polyketide Synthases - genetics; Risk; Rodents; Sequence Deletion; Toxicology; Tumors; Rectal disease; Rodentia; Colorectal cancer; Microflora; Malignant tumor; Carcinogenesis; Colonic disease; Vertebrata; Mammalia; Cancerology; Mouse; Animal; Digestive diseases; Intestinal disease; Colitis; Cancer
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1224820

Inflammation alters host physiology to promote cancer, as seen in colitis-associated colorectal cancer (CRC). Here, we identify the intestinal microbiota as a target of inflammation that affects the progression of CRC. High-throughput sequencing revealed that inflammation modifies gut microbial composition in colitis-susceptible interleukin-10—deficient (Il10⁻ / ⁻) mice. Monocolonization with the commensal Escherichia coli NC101 promoted invasive carcinoma in azoxymethane (AOM)—treated Il10⁻ / ⁻ mice. Deletion of the polyketide synthase (pks) genotoxic island from E. coli NC101 decreased tumor multiplicity and invasion in AOM/Il10⁻ / ⁻ mice, without altering intestinal inflammation. Mucosa-associated pks⁺ E. coli were found in a significantly high percentage of inflammatory bowel disease and CRC patients. This suggests that in mice, colitis can promote tumorigenesis by altering microbial composition and inducing the expansion of microorganisms with genotoxic capabilities.