Neuroprotective Effect of Oxyresveratrol from Smilacis Chinae Rhizome on Amyloid β Protein (25—35)-Induced Neurotoxicity in Cultured Rat Cortical Neurons

• Published in: Biological & pharmaceutical bulletin Vol. 29; no. 12; pp. 2419 - 2424
• Main Authors: Ban, Ju Yeon, Jeon, So-Young, Nguyen, Thi Thuy Ha, Bae, KiHwan, Song, Kyung-Sik, Seonga, Yeon Hee
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.12.2006
• Subjects: Amyloid beta-Peptides - pharmacology; Animals; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - drug effects; cortical neuron; Magnetic Resonance Spectroscopy; Neurons - drug effects; neuroprotection; Neuroprotective Agents - pharmacology; neurotoxicity; oxyresveratrol; Peptide Fragments - pharmacology; Plant Extracts - pharmacology; Rats; Reactive Oxygen Species; Smilacaceae - chemistry; Smilacis chinae; Smilax chinae rhizome; Stilbenes - pharmacology; β-amyloid protein
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.29.2419

We previously reported that Smilacis chinae rhizome inhibits amyloid β protein (25—35) (Aβ (25—35))-induced neurotoxicity in cultured rat cortical neurons. The present study evaluated the neuroprotective effect of oxyresveratrol isolated from Smilacis chinae rhizome against Aβ (25—35)-induced neurotoxicity on cultured rat cortical neurons. Oxyresveratrol over the concentration range of 1—10 μM significantly inhibited 10 μM Aβ (25—35)-induced neuronal cell death, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Oxyresveratrol (10 μM) inhibited 10 μM Aβ (25—35)-induced elevation of cytosolic calcium concentration ([Ca2+]c), which was measured by a fluorescent dye, Fluo-4 AM. Oxyresveratrol (1, 10 μM) also inhibited glutamate release into medium and reactive oxygen species (ROS) generation induced by 10 μM Aβ (25—35). These results suggest that oxyresveratrol prevents Aβ (25—35)-induced neuronal cell damage by interfering with the increase of [Ca2+]c, and then by inhibiting glutamate release and ROS generation. Furthermore, these effects of oxyresveratrol may be associated with the neuroprotective effect of Smilacis chinae rhizome.