Effect of antibiotics, alone and in combination, on Panton–Valentine leukocidin production by a Staphylococcus aureus reference strain

• Published in: Clinical microbiology and infection Vol. 14; no. 4; pp. 384 - 388
• Main Authors: Dumitrescu, O., Badiou, C., Bes, M., Reverdy, M.-E., Vandenesch, F., Etienne, J., Lina, G.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.04.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Acetamides - pharmacology; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial diseases; Bacterial Toxins - biosynthesis; Bacterial Toxins - genetics; Biological and medical sciences; Clindamycin; Clindamycin - pharmacology; Drug Synergism; Drug Therapy, Combination; Exotoxins - biosynthesis; Exotoxins - genetics; fusidic acid; Fusidic Acid - pharmacology; Gene Expression Regulation, Bacterial; Human bacterial diseases; Humans; Infectious diseases; Leukocidins - biosynthesis; Leukocidins - genetics; Linezolid; Medical sciences; Microbial Sensitivity Tests - methods; oxacillin; Oxacillin - pharmacology; Oxazolidinones - pharmacology; Panton–Valentine leukocidin; Pharmacology. Drug treatments; Reference Standards; rifampicin; Rifampin - pharmacology; Staphylococcal infections, streptococcal infections, pneumococcal infections; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - metabolism; Panton–Valentine leukocidin; rifampicin; linezolid; fusidic acid; Clindamycin; oxacillin; Staphylococcus aureus; Linezolid; β-Lactams; Cytokine; Fusidic acid; Penicillin derivatives; Infection; Panton-Valentine leukocidin; Antibiotic; Oxacillin; Rifampicin; Oxazolidinone derivative; Bacteriosis; Bacteria; Micrococcales; Micrococcaceae; Antituberculous agent; Protein synthesis inhibitor; Antibacterial agent; Staphylococcal infection; Oxazole derivatives
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2007.01947.x

The capacity of Staphylococcus aureus strain LUG855 to release Panton–Valentine leukocidin (PVL) in the presence of sub-inhibitory concentrations of anti-staphylococcal drugs was examined. Oxacillin enhanced PVL release 2.5-fold, while clindamycin, linezolid, fusidic acid and rifampicin were inhibitory, and vancomycin, pristinamycin, tetracycline, ofloxacin and co-trimoxazole had no effect. In combination with oxacillin, sub-inhibitory concentrations of clindamycin or rifampicin inhibited PVL induction significantly, linezolid was less inhibitory, and fusidic acid did not inhibit PVL induction by oxacillin. These data support the use of oxacillin in combination with clindamycin, rifampicin or linezolid for the treatment of PVL-positive S. aureus infections.