A PCR–restriction fragment length polymorphism assay to genotype human metapneumovirus

• Published in: Clinical microbiology and infection Vol. 14; no. 1; pp. 91 - 93
• Main Authors: Montes, M., Vicente, D., Esnal, O., Cilla, G., Pérez-Trallero, E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.01.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Amplified Fragment Length Polymorphism Analysis - methods; Biological and medical sciences; Child; Fundamental and applied biological sciences. Psychology; Fusion protein gene; Genetics; Genotype; genotypes; Human metapneumovirus; human metapneumovuris; Humans; identification; lineages; Metapneumovirus - classification; Metapneumovirus - genetics; Metapneumovirus - isolation & purification; Microbiology; Molecular Epidemiology - methods; PCR-RFLP; Viral Fusion Proteins - genetics; Virology; Fusion protein gene; genotypes; identification; human metapneumovuris; PCR-RFLP; lineages; Human; Typing; Genotype; Identification; Infection; Polymerase chain reaction; Microbiological investigation; Restriction fragment length polymorphism; Molecular epidemiology; Genetics; Molecular biology; Fusion protein
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2007.01875.x

Human metapneumovirus (hMPV) genotypes A and B show epidemiological and probably clinical differences. This report describes a fast and simple PCR–restriction fragment length polymorphism (PCR-RFLP) assay, involving digestion of the fusion protein gene with Tsp509I, that allows lineages A1, A2, B1 and B2 to be distinguished. The assay should help in elucidating the epidemiology of hMPV, and possibly in predicting the severity of clinical infection.