DAB2IP Downregulation Enhances the Proliferation and Metastasis of Human Gastric Cancer Cells by Derepressing the ERK1/2 Pathway

DAB2IP (DOC2/DAB2 interactive protein) is downregulated in several cancer types, and its downregulation is involved in tumor cell proliferation, apoptosis, metastasis, and epithelial-mesenchymal transition (EMT). We aimed to investigate the potential role of DAB2IP in the development and progression...

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Published inGastroenterology research and practice Vol. 2018; no. 2018; pp. 1 - 10
Main Authors He, Songbing, Zhu, Xinguo, Shi, Weiqiang, Zhan, Shenghua, Shang, Zengfu, Lu, Ting, Yao, Yizhou, Sun, Liang, Pan, Guofeng
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2018
Hindawi
Hindawi Limited
Wiley
Subjects
Apoptosis
Cancer
Cancer cells
Cancer metastasis
Cancer prevention
Cell growth
Colorectal cancer
Development and progression
Gastric cancer
Gene expression
Genetic engineering
Immunoglobulins
Immunohistochemistry
Medical research
Messenger RNA
Metastasis
Penicillin G
Prostate cancer
Proteins
RNA
Stem cells
Stomach cancer
Tumors
China
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Summary:DAB2IP (DOC2/DAB2 interactive protein) is downregulated in several cancer types, and its downregulation is involved in tumor cell proliferation, apoptosis, metastasis, and epithelial-mesenchymal transition (EMT). We aimed to investigate the potential role of DAB2IP in the development and progression of gastric cancer. DAB2IP levels were analyzed in human gastric cancer and adjacent normal tissues by Western blots and immunohistochemistry. Potential roles of DAB2IP in regulating gastric cancer cell growth and metastasis were examined by genetic manipulation in vitro. The molecular signaling was determined to understand the mechanisms of observed DAB2IP effects. DAB2IP level is lower in gastric cancer tissues as compared to paired normal tissues. Knockdown of DAB2IP enhanced gastric cancer cell growth and metastasis in vitro and promoted EMT progress at both protein and mRNA levels. Silencing DAB2IP activated extracellular signal-regulated kinase 1/2 (ERK1/2) pathway, and the enhanced proliferation and migration ability induced by DAB2IP knockdown were reduced after incubation with U0126 in SGC7901 gastric cancer cells. Inhibition of DAB2IP enhances gastric cancer cell growth and metastasis through targeting the ERK1/2 signaling, indicating that it may serve as a potential target for treatment of gastric cancer.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Academic Editor: Niccola Funel
ISSN:1687-6121
1687-630X
DOI:10.1155/2018/2968252