Product of the Lactococcus lactis gene required for malolactic fermentation is homologous to a family of positive regulators

• Published in: Journal of Bacteriology Vol. 171; no. 6; pp. 3108 - 3114
• Main Authors: Renault, P, Gaillardin, C, Heslot, H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.1989
• Subjects: Amino Acid Sequence; Bacteriology; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA, Bacterial - genetics; FERMENTACION; FERMENTATION; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; Genes, Regulator; Genetic Complementation Test; Genetics; Lactates - biosynthesis; Lactic Acid; Lactococcus lactis; Lactococcus lactis - genetics; Life Sciences; MALATE; Malates - metabolism; MALATOS; Microbiology; Microbiology and Parasitology; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; PROTEINAS; PROTEINE; Recombinant Fusion Proteins - genetics; STREPTOCOCCUS; Lactococcus lactis; Nucleotide sequence; Gel electrophoresis; Induction; Gene product; Gene expression; Fermentation; Genetic transfer; Malolactic fermentation; Streptococcaceae; Regulation(control); Gene; DNA; Restriction map; Bacteria; Micrococcales; Isolation; Molecular cloning; Southern blotting; Aminoacid sequence; CARTE GENIQUE; SEQUENCE NUCLEOTIQUE; INDUCTION; LACTOCOCCUS LACTIS; ACIDE LACTIQUE
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.171.6.3108-3114.1989

Malolactic fermentation is a secondary fermentation that many lactic acid bacteria can carry out when L-malate is present in the medium. The activation of the malolactic system in Lactococcus lactis is mediated by a locus we call meleR. Induction of the genes necessary to perform malolactic fermentation occurs only in bacteria with a functional copy of mleR. The mleR gene consists of one open reading frame capable of coding for a protein with a calculated molecular mass of 33,813 daltons. The amino acid sequence of the predicted MIeR gene product is homologous to that of positive activators in gram-negative bacteria: LysR, IlvY gene products of Escherichia coli, MetR, CysB of Salmonella typhimurium, AmpR of Enterobacter cloacae, NodD of Rhizobium sp., and TrpI of Pseudomonas aeruginosa