Microcontact Imprinted Plasmonic Nanosensors: Powerful Tools in the Detection of Salmonella paratyphi

• Published in: Sensors (Basel, Switzerland) Vol. 17; no. 6; p. 1375
• Main Authors: Perçin, Işık, Idil, Neslihan, Bakhshpour, Monireh, Yılmaz, Erkut, Mattiasson, Bo, Denizli, Adil
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.06.2017; MDPI
• Subjects: ">l-histidine methyl ester; Basic Medicine; Chips; Ellipsometry; Engineering and Technology; Histidine; Histidine - analogs & derivatives; Identification methods; Imprinted polymers; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Methacrylates; Microbiology in the medical area; Microcontact imprinting; Microorganisms; Mikrobiologi inom det medicinska området; Molecular Imprinting; N-methacryloyl; N-methacryloyl-L-histidine methyl ester; Nano Technology; Nanosensors; Nanostructures; Nanoteknik; Polymers; Salmonella; Salmonella paratyphi; Salmonella paratyphi A; Scanning electron microscopy; Sensors; SPR biosensor; Staphylococcus aureus; Surface Plasmon Resonance; Teknik; Salmonella paratyphi; ">l-histidine methyl ester; microcontact imprinting; N-methacryloyl; SPR biosensor
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s17061375

Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting . For this purpose, the stamps of the target microorganism were prepared and then, microcontact -imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time detection was performed within the range of 2.5 × 10⁶-15 × 10⁶ CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as , and . The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of was achieved using these SPR sensor with a detection limit of 1.4 × 10⁶ CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.