In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure

Single embryo culture is useful for assessing the developmental competence of an embryo in detail. Recently, a device made of poly(dimethylsiloxane) (PDMS), which is biocompatible and nontoxic, has been widely used for culture various types of cells. However, PDMS plates are porous, causing the seri...

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Published inInternational journal of biomaterials Vol. 2018; no. 2018; pp. 1 - 7
Main Authors Kishi, Masao, Taguchi, Yoshitomo, Taniguchi, Shunji, Kato, Nobuhiro, Iwamoto, Daisaku, Saeki, Kazuhiro
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2018
Hindawi
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Biocompatibility
Biology
Cell culture
Curing
Dimethylpolysiloxane
Embryonic development
Embryos
Humidity
In vitro fertilization
Low pressure
Plates
Polydimethylsiloxane
Porosity
Pressure
Silicone resins
Sperm
United States > US
Japan
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Summary:Single embryo culture is useful for assessing the developmental competence of an embryo in detail. Recently, a device made of poly(dimethylsiloxane) (PDMS), which is biocompatible and nontoxic, has been widely used for culture various types of cells. However, PDMS plates are porous, causing the serious osmolality increment of the medium (over 600 mOsm/kg from Day 4 to Day 7). Here, we report that curing the PDMS under low pressure (LP-PDMS) greatly reduced the porosity, resulting in a constant osmolality of the medium. The blastocyst rate of single bovine embryos cultured with LP-PDMS microwell (MW) plates was the same as that of group-cultured embryos (25 embryos/50 μl droplet; control, P>0.05). These results indicate that MWs on a plate made of PDMS cured under low pressure can be successfully used for individual embryo culture.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Academic Editor: Alexander Seifalian
ISSN:1687-8787
1687-8795
DOI:10.1155/2018/7546986