UPLC-Tandem Mass Spectrometry Method for Simultaneous Determination of Fluoxetine, Risperidone, and Its Active Metabolite 9-Hydroxyrisperidone in Plasma: Application to Pharmacokinetics Study in Rats

• Published in: Journal of analytical methods in chemistry Vol. 2017; no. 2017; pp. 1 - 10
• Main Authors: Ezzeldin, Essam, Tammam, Marwa, Abo Talib, Nisreen F.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2017; Hindawi; Hindawi Limited; Wiley
• Subjects: Accuracy; Analysis; Bioavailability; Calibration; Chemical properties; Chromatography; Drug dosages; Ions; Laboratory animals; Mass spectrometry; Methods; NMR; Nuclear magnetic resonance; Pharmaceuticals; Pharmacokinetics; Pharmacological research; Plasma; Psychotropic drugs; Quality control; Risperidone; Scientific imaging; Studies; Vortices
• ISSN: 2090-8865; 2090-8873
• DOI: 10.1155/2017/5187084

Risperidone (RIS) is used as an antipsychotic drug alone or with other drugs, like fluoxetine (FLX). A simple method was developed and validated for the determination of both RIS and its metabolite 9-hydroxyrisperidone (9-OH-RIS), FLX, and olanzapine (OLA) as an internal standard in rat’s plasma using UPLC-MS/MS. FLX, RIS, 9-OH-RIS, and OLA were purified using acetonitrile as a protein precipitating agent. Separation was performed on an ACQUITY™ “UPLC BEH™” C18 column (50 mm × 2.1 mm i.d., 1.7 μm; Waters Corp., USA). The ranges of the calibration curves were 1.0–1000.0 ng/mL for FLX and 0.2–1000.0 ng/mL for RIS and 9-OH-RIS. Linearity, recovery, precision, and stability were within the acceptable range. This method is rapid, fast, and precise for the determination of RIS and FLX in plasma and is applicable in pharmacokinetic studies.