Transgenerational effects of binge drinking in a primate model: implications for human health

Objective To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development. Design Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating. Set...

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Bibliographic Details
Published inFertility and sterility Vol. 103; no. 2; pp. 560 - 569
Main Authors VandeVoort, Catherine A., Ph.D, Grimsrud, Kristin N., D.V.M., Ph.D, Midic, Uros, Ph.D, Mtango, Namdori, Ph.D, Latham, Keith E., Ph.D
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.2015
Subjects
Animals
Binge Drinking - complications
Binge Drinking - pathology
cDNA array
Cohort Effect
Cumulus cells
Ethanol - administration & dosage
Ethanol - toxicity
Female
fetal alcohol syndrome
granulosa cells
Health Status
Humans
Internal Medicine
Macaca mulatta
Models, Animal
Obstetrics and Gynecology
Oocytes - drug effects
Oocytes - pathology
Pregnancy
Pregnancy Rate - trends
reproduction
transcriptome
cDNA array
fetal alcohol syndrome
granulosa cells
transcriptome
Cumulus cells
reproduction
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Summary:Objective To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development. Design Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating. Setting National primate research center. Animal(s) Adult female rhesus monkeys. Intervention(s) Binge levels of ethanol, given twice weekly for 6 months before a standard in vitro fertilization cycle with or without embryo culture. With in vivo development, ethanol treatment continued until pregnancy was identified. Main Outcome Measure(s) Oocyte and cumulus/granulosa cell gene expression, embryo development to blastocyst, and pregnancy rate. Result(s) Embryo development in vitro was reduced; changes were found in oocyte and cumulus cell gene expression; and spontaneous abortion during very early gestation increased. Conclusion(s) This study provides evidence that binge drinking can affect the developmental potential of oocytes even after alcohol consumption has ceased.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2014.10.051