Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA

The nuclear gene PIF of Saccharomyces cerevisiae is required for both repair of mitochondrial DNA (mtDNA) and recognition of a recombinogenic signal characterized by a 26‐bp palindromic AT sequence in the ery region of mtDNA. This gene has been cloned in yeast by genetic complementation of pif mutan...

Full description

Saved in:
Bibliographic Details
Published inThe EMBO journal Vol. 6; no. 5; pp. 1441 - 1449
Main Authors Foury, F., Lahaye, A.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group 01.05.1987
Subjects
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Biotechnology
Cloning, Molecular
DNA
DNA Repair
DNA Restriction Enzymes
DNA, Mitochondrial - genetics
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Genes
Genes, Fungal
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
nucleotide sequence
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Sequence Homology, Nucleic Acid
Fungi
Recombination
Yeast
Gene
Primary structure
Ascomycetes
Mitochondrial DNA
Molecular cloning
Repair
Saccharomyces cerevisiae
Thallophyta
Online AccessGet full text

Cover

More Information
Summary:The nuclear gene PIF of Saccharomyces cerevisiae is required for both repair of mitochondrial DNA (mtDNA) and recognition of a recombinogenic signal characterized by a 26‐bp palindromic AT sequence in the ery region of mtDNA. This gene has been cloned in yeast by genetic complementation of pif mutants. Its chromosomal disruption does not destroy the genetic function of mitochondria. The nucleotide sequence of the 3.5‐kb insert from a complementing plasmid reveals an open reading frame encoding a potential protein of 857 amino acids and Mr = 97,500. An ATP‐binding domain is present in the central part of the gene and in the carboxy‐terminal region a putative DNA‐binding site is present. Its alpha helix‐turn‐alpha helix motif is found in DNA‐binding proteins such as lambda and lactose repressors which recognize symmetric sequences. Significant amino acid homology is observed with yeast RAD3 and E. coli UvrD (helicase II) proteins which are required for excision repair of damaged DNA.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1987.tb02385.x