Physical and functional association of SU(VAR)3-9 and HDAC1 in Drosophila
Modification of histones can have a dramatic impact on chromatin structure and function. Acetylation of lysines within the N‐terminal tail of the histone octamer marks transcriptionally active regions of the genome whereas deacetylation seems to play a role in transcriptional silencing. Recently, th...
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Published in | EMBO reports Vol. 2; no. 10; pp. 915 - 919 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.10.2001
Blackwell Publishing Ltd Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Modification of histones can have a dramatic impact on chromatin structure and function. Acetylation of lysines within the N‐terminal tail of the histone octamer marks transcriptionally active regions of the genome whereas deacetylation seems to play a role in transcriptional silencing. Recently, the methylation of the histone tails has also been shown to be important for transcriptional regulation and chromosome structure. Here we show by immunoaffinity purification that two activities important for chromatin‐mediated gene silencing, the histone methyltransferase SU(VAR)3‐9 and the histone deacetylase HDAC1, associate in vivo. The two activities cooperate to methylate pre‐acetylated histones. Both enzymes are modifiers of position effect variegation and interact genetically in flies. We suggest a model in which the concerted histone deacetylation and methylation by a SU(VAR)3‐9/HDAC1‐containing complex leads to a permanent silencing of transcription in particular areas of the genome. |
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Bibliography: | ArticleID:EMBR311 istex:8A834F8E0AC76DE04C1D28305015B3A0CD83A1CC ark:/67375/WNG-T5M6QRWW-7 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author. Tel: +49 89 5996 435; Fax: +49 89 5996 425; E-mail: axel.imhof@mol-bio.med.uni-muenchen.de |
ISSN: | 1469-221X 1469-3178 |
DOI: | 10.1093/embo-reports/kve210 |