Neuronal differentiation signals are controlled by nerve growth factor receptor/Trk binding sites for SHC and PLC gamma

• Published in: The EMBO journal Vol. 13; no. 7; pp. 1585 - 1590
• Main Authors: Obermeier, A., Bradshaw, R.A., Seedorf, K., Choidas, A., Schlessinger, J., Ullrich, A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.04.1994
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cell Differentiation - physiology; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Molecular Sequence Data; Nerve Growth Factors - pharmacology; Neurons - physiology; PC12 Cells - physiology; Phosphatidylinositol 3-Kinases; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Platelet-Derived Growth Factor - pharmacology; Protein-Tyrosine Kinases - metabolism; Receptors, Nerve Growth Factor - metabolism; Recombinant Proteins - metabolism; Signal Transduction - physiology; Transfection; Type C Phospholipases - metabolism; Rat; Adrenal glands; Enzyme; Transferases; Rodentia; Nerve growth factor; Esterases; Phosphoric diester hydrolases; Cell differentiation; In vitro; Mechanism; Phospholipase C; Signal transduction; Vertebrata; Membrane receptor; Cell line; Mammalia; Neuron; Polypeptide; Hydrolases; Protein-tyrosine kinase; Growth factor
• ISSN: 0261-4189; 1460-2075
• DOI: 10.1002/j.1460-2075.1994.tb06421.x

Differentiation and survival of neuronal cell types requires the action of neurotrophic polypeptides such as nerve growth factor (NGF). In the central and peripheral nervous system and the phaeochromocytoma cell model PC12, NGF exerts its effects through the activation of the signalling capacity of Trk, a receptor tyrosine kinase (RTK) which upon interaction with NGF becomes phosphorylated on tyrosines and thereby acquires the potential to interact with signal‐transducing proteins such as phospholipase C‐gamma (PLC gamma), phosphatidylinositol‐3′‐kinase (PI3′‐K) and SHC. Mutagenesis of the specific binding sites for these src homology 2 (SH2) domain‐containing substrates within the Trk cytoplasmic domain suggests a non‐essential function of PI3′‐K and reveals a major role for the signal controlled by the SHC binding site at tyrosine 490 and a co‐operative function of the PLC gamma‐mediated pathway for neuronal differentiation of PC12 cells.