Occurrence of chronic endometritis in different types of human adenomyosis

• Published in: Reproductive medicine and biology Vol. 21; no. 1; pp. e12421 - n/a
• Main Authors: Khan, Khaleque N., Fujishita, Akira, Ogawa, Kanae, Koshiba, Akemi, Mori, Taisuke, Itoh, Kyoko, Nakashima, Masahiro, Kitawaki, Jo
• Format: Journal Article
• Language: English
• Published: Japan John Wiley & Sons, Inc 01.01.2022; John Wiley and Sons Inc; Wiley
• Subjects: Antigens; Biopsy; CD138; CD68; Endometriosis; Endometritis; Endometrium; extrinsic adenomyosis; Fertility; focal/diffuse adenomyosis; Histology; Hysterectomy; Infections; Infertility; Infiltration; intrinsic adenomyosis; Macrophages; Magnetic resonance imaging; Monoclonal antibodies; Original; Plasma cells; Stroma; Surgery; Syndecan; Vagina; extrinsic adenomyosis; focal/diffuse adenomyosis; CD138; CD68; intrinsic adenomyosis
• ISSN: 1445-5781; 1447-0578
• DOI: 10.1002/rmb2.12421

Purpose Human adenomyosis has an adverse effect on female fertility. Exact mechanistic basis is still unclear. We investigated the occurrence of chronic endometritis (CE) in different types of human adenomyosis. Methods This is a prospective non‐randomized observational study enrolling patients with focal (n = 30), diffuse (n = 26), intrinsic (n = 23), and extrinsic (n = 10) adenomyosis. Endometrial biopsy samples were collected from hysterectomy specimens. Immunohistochemical analysis was performed using antibody against CD68 (Mφ marker) with biopsy samples of intrinsic/extrinsic adenomyosis and CD138 (Syndecan‐1), a marker of plasma cells, in all biopsy samples. Results In GnRHa‐untreated groups, a higher trend in the occurrence of CE, as characterized by infiltration of ≥1 plasma cells in endometrial stroma, was found in women with focal (58.8%, p = 0.0849) and diffuse adenomyosis (60.0%, p = 0.0841) comparing to control women (10.0%). In women with focal adenomyosis, ipsilateral side showed a significantly higher occurrence of CE (58.8%) than on the contralateral side (11.7%) (p = 0.043). Tissue infiltration of macrophages in endometria was significantly higher in intrinsic than in extrinsic adenomyosis (p = 0.03) without showing any significant difference in the occurrence of CE between these two variants of adenomyosis. Conclusion A variable occurrence of CE in different types of adenomyosis may be involved in adverse reproductive outcome.