植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响
目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR法分别检测HPV16/18 E6/E7 mRNA,采用Western blotting法检测E6/E7蛋白。结果实验1、2、3、4组同一型别细胞中E6/E7 mRNA及蛋白相对表达...
Saved in:
| Published in | 山东医药 Vol. 56; no. 7; pp. 10 - 12 |
|---|---|
| Main Author | |
| Format | Magazine Article |
| Language | Chinese |
| Published |
天津医科大学总医院,天津,300052
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-266X |
| DOI | 10.3969/j.issn.1002-266X.2016.07.004 |
Cover
| Abstract | 目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR法分别检测HPV16/18 E6/E7 mRNA,采用Western blotting法检测E6/E7蛋白。结果实验1、2、3、4组同一型别细胞中E6/E7 mRNA及蛋白相对表达量低于对照组,且实验1、2、3、4组E6/E7 mRNA及蛋白相对表达量依次降低（P均〈0.05）。各实验组内,Caski细胞中E6/E7 mRNA相对表达量低于Hela细胞（P均〈0.05）。各实验组内同一型别细胞中,E6 mRNA相对表达量低于E7 mRNA（P均〈0.05）。结论植酸酮可下调人宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达,且作用呈剂量依赖性;植酸酮对HPV16 E6/E7 mRNA的抑制作用强于HPV18 E6/E7 mRNA,并可能以抑制E6 mRNA表达为主导。 |
|---|---|
| AbstractList | R711.74; 目的：观察植酸酮对宫颈癌细胞株中HPV16／18 E6／E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18），将所有细胞分成实验1、2、3、4组和对照组，每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58．6、117、586、5860 mg／L植酸酮的培养液，对照组加入不含植酸酮的培养液。各组培养72 h后，采用real-time PCR法分别检测HPV16／18 E6／E7 mRNA，采用Western blotting法检测E6／E7蛋白。结果实验1、2、3、4组同一型别细胞中E6／E7 mRNA及蛋白相对表达量低于对照组，且实验1、2、3、4组E6／E7 mRNA及蛋白相对表达量依次降低（P均＜0．05）。各实验组内，Caski细胞中E6／E7 mRNA相对表达量低于Hela细胞（P均＜0．05）。各实验组内同一型别细胞中，E6 mRNA相对表达量低于E7 mRNA（P均＜0．05）。结论植酸酮可下调人宫颈癌细胞株中HPV16／18 E6／E7 mRNA及蛋白表达，且作用呈剂量依赖性；植酸酮对HPV16 E6／E7 mRNA的抑制作用强于HPV18 E6／E7 mRNA，并可能以抑制E6 mRNA表达为主导。 目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR法分别检测HPV16/18 E6/E7 mRNA,采用Western blotting法检测E6/E7蛋白。结果实验1、2、3、4组同一型别细胞中E6/E7 mRNA及蛋白相对表达量低于对照组,且实验1、2、3、4组E6/E7 mRNA及蛋白相对表达量依次降低（P均〈0.05）。各实验组内,Caski细胞中E6/E7 mRNA相对表达量低于Hela细胞（P均〈0.05）。各实验组内同一型别细胞中,E6 mRNA相对表达量低于E7 mRNA（P均〈0.05）。结论植酸酮可下调人宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达,且作用呈剂量依赖性;植酸酮对HPV16 E6/E7 mRNA的抑制作用强于HPV18 E6/E7 mRNA,并可能以抑制E6 mRNA表达为主导。 |
| Abstract_FL | Objective To observe the effects of phytic acid ketone on expression of HPV 16/18 E6/E7 mRNA and protein in human cervical cancer cells.Methods The Caski cells ( containing HPV16 ) and Hela cells ( containing HPV18) were cultivated in vitro and then divided into the experimental groups 1, 2, 3, 4 and the control group ( each group contained Caski cell line and Hela cell line).The experimental groups 1, 2, 3, 4 were respectively treated with dif-ferent concentrations of phytic acid ketone (58.6 mg/L, 117 mg/L, 586 mg/L, 5 860 mg/L) for 72 h.The levels of HPV16/18 E6/E7 mRNA were determined with real-time PCR.Western blotting was used to measure the expression levels of E6 and E7 proteins.Results E6/E7 mRNA and protein expression in the same type of cells of the experimental groups 1, 2, 3 and 4 was lower than that of the control group, and the E6/E7 mRNA and protein expression showed a decreasing tendency in the experimental groups 1, 2, 3 and 4 (all P<0.05).In each experimental group, E6/E7 mRNA expression in Caski cells was lower than that in Hela cells (all P<0.05).In the same type of cells of the experiential groups, the E6 mRNA expression was lower than that of E7 mRNA (all P<0.05).Conclusions Phytic acid ketone could down-regulate the expression of E6/E7 mRNA and protein in human cervical cancer lines, which was in a dose-dependent manner.The inhibitory effect of phytic acid ketone on HPV16 E6/E7 mRNA was stronger than that on HPV18 E6/E7 mRNA and the suppression of E6 probably played a leading role. |
| Author | 王侃 高晓丽 田文艳 岳天孚 |
| AuthorAffiliation | 天津医科大学总医院,天津300052 |
| AuthorAffiliation_xml | – name: 天津医科大学总医院,天津,300052 |
| Author_FL | TIAN Wenyan GAO Xiaoli WANG Kan YUE Tianfu |
| Author_FL_xml | – sequence: 1 fullname: WANG Kan – sequence: 2 fullname: GAO Xiaoli – sequence: 3 fullname: TIAN Wenyan – sequence: 4 fullname: YUE Tianfu |
| Author_xml | – sequence: 1 fullname: 王侃 高晓丽 田文艳 岳天孚 |
| BookMark | eNo9j01LAkEAhudgkFr_QaJDl11nZqfZmaOIZSAVIdFNZtcdP8ixXCI8mxGVdOhkQVR0iCKjmxH6a1p3_RdNGJ1eeHl4PxIgpprKA2AZQdPilKfrZs33lYkgxAamdM_EEFET2iaEJAbi__48SPh-XZsY2TwOCpOn3rQ7nHYHwftnMHidPp6F_cvw6zTq3E3uX76Hb_ntXUTTiKVyNJ2zU42dzUxwdR7dXoT9UfTwHI3H4c1JMPoIrnsLYE6Kfd9b_NMkKK7litm8Udha38hmCoZLITEcJKDFynoBtbgrbI_ZjoOIFIRJj0qOuGdRSSzEGGFwVSMIliURDDrQdRi2kmBlFnsslBSqUqo3j1pKF5b8qlDldvv3OrT1R40uzVC32lSVw5qGD1q1hmi1S5QyTDhG2PoBMIlw8g |
| ClassificationCodes | R711.74 |
| ContentType | Magazine Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.1002-266X.2016.07.004 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Effect of phytic acid ketone on expression of HPV16/18 E6/E7 mRNA and protein in human cervical cancer cell line |
| DocumentTitle_FL | Effect of phytic acid ketone on expression of HPV16/18 E6/E7 mRNA and protein in human cervical cancer cell line |
| EndPage | 12 |
| ExternalDocumentID | shandyy201607004 668249212 |
| GroupedDBID | -05 2B. 2C~ 2RA 5XA 5XF 92F 92I 92L ABDBF ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CIEJG CQIGP CW9 EOJEC OBODZ TCJ TGQ U1G U5O W91 ~WA 4A8 93N ABJNI ACUHS PSX |
| ID | FETCH-LOGICAL-c604-b1a038d042639ca7e87bb14fa48fe6f919e36f4318848059ca10df4a80b0cb823 |
| ISSN | 1002-266X |
| IngestDate | Thu May 29 04:06:26 EDT 2025 Wed Feb 14 10:20:35 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 7 |
| Keywords | 人乳头瘤病毒16 E6 protein human papillomavirus 18 宫颈癌 Hela细胞株 人乳头瘤病毒18 E7 protein Hela cell line E6蛋白 E7蛋白 Caski细胞株 phytic acid ketone Caski cell line human papillomavirus 16 cervi-cal carcinoma 植酸酮 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c604-b1a038d042639ca7e87bb14fa48fe6f919e36f4318848059ca10df4a80b0cb823 |
| Notes | Objective To observe the effects of phytic acid ketone on expression of HPV 16 /18 E6 / E7 mRNA and protein in human cervical cancer cells. Methods The Caski cells（ containing HPV16） and Hela cells（ containing HPV18） were cultivated in vitro and then divided into the experimental groups 1,2,3,4 and the control group（ each group contained Caski cell line and Hela cell line）. The experimental groups 1,2,3,4 were respectively treated with different concentrations of phytic acid ketone（ 58. 6 mg / L,117 mg / L,586 mg / L,5 860 mg / L） for 72 h. The levels of HPV16 /18 E6 / E7 mRNA were determined with real-time PCR. Western blotting was used to measure the expression levels of E6 and E7 proteins. Results E6 / E7 mRNA and protein expression in the same type of cells of the experimental groups1,2,3 and 4 was lower than that of the control group,and the E6 / E7 mRNA and protein expression showed a decreasing tendency in the experimental groups 1,2,3 and 4（ all P〈0. 05）. In each experimental group,E6 / E7 mRNA expres |
| PageCount | 3 |
| ParticipantIDs | wanfang_journals_shandyy201607004 chongqing_primary_668249212 |
| PublicationCentury | 2000 |
| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 山东医药 |
| PublicationTitleAlternate | Shandong Medical Journal |
| PublicationYear | 2016 |
| Publisher | 天津医科大学总医院,天津,300052 |
| Publisher_xml | – name: 天津医科大学总医院,天津,300052 |
| SSID | ssj0042179 ssib001104105 ssib017477535 ssib051371736 ssib002262813 |
| Score | 1.3045241 |
| Snippet | 目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860... R711.74; 目的：观察植酸酮对宫颈癌细胞株中HPV16／18 E6／E7... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 10 |
| SubjectTerms | Caski细胞株 E6蛋白 E7蛋白 Hela细胞株 人乳头瘤病毒16 人乳头瘤病毒18 宫颈癌 植酸酮 |
| Title | 植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响 |
| URI | http://lib.cqvip.com/qk/92062X/201607/668249212.html https://d.wanfangdata.com.cn/periodical/shandyy201607004 |
| Volume | 56 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Pa9RAFA-1BfGkomKtygqd07I1_zbz5jjJzlIEi5QqvS1JNmkvbq1tD-1VK6KWHjxVQVQ8iGLFW0XbT-N2tye_gu9NsrthKVKFJfsy8_J7b-ZlMm8mkzeGMZnwKLR4klaaMc1WCTuuRFZkVmwIU5Fy2872jLw9403fdW_NV-dHTi0XVi2trUZT8cax35X8j1UxDe1KX8n-g2X7oJiANNoXj2hhPJ7Ixkx5TKIzWGNKMKgyH3JCKqbwWGe-0IRi0qcsaTNAHs4EsgVE-D4Djylg4DChNKDJpGTKJTRZK0_fuYcDb4VQAYO6BWU1OFO8fH92RpZJBtQZSAISiOjnMvwapUiLSSDCV_SjLMnApauQwbeIEA4Wo-gp61xL52pVRKClBKQxqVvD4vXuFoIEpcW6JAGcQY6gogugkqFCKCaD82vFiwWmmZrFY8A1PipfQEFdbEzQlekymdVqDctRnDWxhmdNCuwe85GoFsrAmUTBVo-H55DSI2YwNU-fWZDyaD07OAbTDhz9LrXQwVAPZGe7N_baFi90IPka38wVyRaYD3dyjvCE7uQIcKoPSMsUs0C02X7OQ2HEV-hFzPq6rSMK6gi6YzbHsfyoMSb9ml8f-NA4QLcKnQD65zYMfL6q5dDCDa_n7rg4phV6SUGux2ljMlfy5t9UpFgmi0uthWX00PQHc600bC0UfLu5c8bZXpD1ksya2HljZGPxgjF7-GHraHPvaHO3_fV7e_fz0funnZ0XnR9Puo_eHL799GvvS0k3jt8_ty0oKfpXvEQNotTeftZ9_byzs99997F7cNB59bi9_639cuuiMVdXc8F0Jd-CpBJ7pouPrdB0oEnzDI6IQ54AjyLLTUMX0sRLhSUSx0vRBwdwAQcqcWiZzdQNwYzMOALbuWSMtpZayWWj5HEzdmN0_h1w3SR1IKo6EUTca3JIhV0dNyb69dF4kEWaaXgeUERPyx43buQ11MifPyuNYYteOQHPhHFm0BquGqOrD9eSa-hVr0bX8_vgD4LDndg |
| linkProvider | EBSCOhost |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E6%A4%8D%E9%85%B8%E9%85%AE%E5%AF%B9%E5%AE%AB%E9%A2%88%E7%99%8C%E7%BB%86%E8%83%9E%E6%A0%AA%E4%B8%AD+HPV16%EF%BC%8F18+E6%EF%BC%8FE7+mRNA+%E5%8F%8A%E8%9B%8B%E7%99%BD%E8%A1%A8%E8%BE%BE%E7%9A%84%E5%BD%B1%E5%93%8D&rft.jtitle=%E5%B1%B1%E4%B8%9C%E5%8C%BB%E8%8D%AF&rft.au=%E7%8E%8B%E4%BE%83&rft.au=%E9%AB%98%E6%99%93%E4%B8%BD&rft.au=%E7%94%B0%E6%96%87%E8%89%B3&rft.au=%E5%B2%B3%E5%A4%A9%E5%AD%9A&rft.date=2016-01-01&rft.pub=%E5%A4%A9%E6%B4%A5%E5%8C%BB%E7%A7%91%E5%A4%A7%E5%AD%A6%E6%80%BB%E5%8C%BB%E9%99%A2%2C%E5%A4%A9%E6%B4%A5%2C300052&rft.issn=1002-266X&rft.issue=7&rft.spage=10&rft.epage=12&rft_id=info:doi/10.3969%2Fj.issn.1002-266X.2016.07.004&rft.externalDocID=shandyy201607004 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F92062X%2F92062X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fshandyy%2Fshandyy.jpg |