植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响

目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR法分别检测HPV16/18 E6/E7 mRNA,采用Western blotting法检测E6/E7蛋白。结果实验1、2、3、4组同一型别细胞中E6/E7 mRNA及蛋白相对表达...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 7; pp. 10 - 12
Main Author 王侃 高晓丽 田文艳 岳天孚
Format Magazine Article
LanguageChinese
Published 天津医科大学总医院,天津,300052 2016
Subjects
Caski细胞株
E6蛋白
E7蛋白
Hela细胞株
人乳头瘤病毒16
人乳头瘤病毒18
宫颈癌
植酸酮
人乳头瘤病毒16
E6 protein
human papillomavirus 18
宫颈癌
Hela细胞株
人乳头瘤病毒18
E7 protein
Hela cell line
E6蛋白
E7蛋白
Caski细胞株
phytic acid ketone
Caski cell line
human papillomavirus 16
cervi-cal carcinoma
植酸酮
Online AccessGet full text
ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.07.004

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Summary:目的观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株（含HPV16）与Hela细胞株（含HPV18）,将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5 860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR法分别检测HPV16/18 E6/E7 mRNA,采用Western blotting法检测E6/E7蛋白。结果实验1、2、3、4组同一型别细胞中E6/E7 mRNA及蛋白相对表达量低于对照组,且实验1、2、3、4组E6/E7 mRNA及蛋白相对表达量依次降低（P均〈0.05）。各实验组内,Caski细胞中E6/E7 mRNA相对表达量低于Hela细胞（P均〈0.05）。各实验组内同一型别细胞中,E6 mRNA相对表达量低于E7 mRNA（P均〈0.05）。结论植酸酮可下调人宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达,且作用呈剂量依赖性;植酸酮对HPV16 E6/E7 mRNA的抑制作用强于HPV18 E6/E7 mRNA,并可能以抑制E6 mRNA表达为主导。
Bibliography:Objective To observe the effects of phytic acid ketone on expression of HPV 16 /18 E6 / E7 mRNA and protein in human cervical cancer cells. Methods The Caski cells（ containing HPV16） and Hela cells（ containing HPV18） were cultivated in vitro and then divided into the experimental groups 1,2,3,4 and the control group（ each group contained Caski cell line and Hela cell line）. The experimental groups 1,2,3,4 were respectively treated with different concentrations of phytic acid ketone（ 58. 6 mg / L,117 mg / L,586 mg / L,5 860 mg / L） for 72 h. The levels of HPV16 /18 E6 / E7 mRNA were determined with real-time PCR. Western blotting was used to measure the expression levels of E6 and E7 proteins. Results E6 / E7 mRNA and protein expression in the same type of cells of the experimental groups1,2,3 and 4 was lower than that of the control group,and the E6 / E7 mRNA and protein expression showed a decreasing tendency in the experimental groups 1,2,3 and 4（ all P〈0. 05）. In each experimental group,E6 / E7 mRNA expres
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.07.004