Characterization of rumen bacterial strains isolated from enrichments of rumen content in the presence of propolis

• Published in: World journal of microbiology & biotechnology Vol. 30; no. 11; pp. 2917 - 2926
• Main Authors: de Aguiar, Sílvia Cristina, Zeoula, Lucia Maria, do Prado, Odimari Pricila Pires, Arcuri, Pedro Braga, Forano, Evelyne
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.11.2014; Springer Netherlands; Springer Nature B.V; Springer Verlag
• Subjects: Alcohol; Analysis; Animals; Anti-Bacterial Agents - metabolism; antibacterial properties; Antimicrobial agents; Applied Microbiology; Bacillus; Bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - growth & development; Bacterial Typing Techniques; Biochemistry; Biological; Biomedical and Life Sciences; Biotechnology; Brazil; carboxymethylcellulose; Cattle; Clostridium bifermentans; Cluster Analysis; cows; Degradation; Digestive system; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; E coli; Enrichment; Environmental Engineering/Biotechnology; Enzymes; Escherichia coli; Life Sciences; Microbiology; Microbiology and Parasitology; Mitsuokella jalaludinii; Molecular Sequence Data; Natural products; Nutrition; Original Paper; phenolic compounds; phenotype; Phylogeny; Propolis; Propolis - metabolism; proteolysis; ribosomal RNA; RNA, Ribosomal, 16S - genetics; rumen; Rumen - microbiology; rumen bacteria; ruminant nutrition; Ruminantia; Selenomonas ruminantium; Sequence Analysis, DNA; starch; Strain; Streptococcus; Studies; sugars; Brazil; Antibacterial activity; Rumen bacteria; Phenolic compounds; Propolis; antibacterial activity; phenolic compounds; propolis; rumen bacteria
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-014-1719-8

Propolis presents many biological properties, including antibacterial activities, and has been proposed as an additive in ruminant nutrition. Twenty bacterial strains, previously isolated from enrichments of Brazilian cow rumen contents in the presence of different propolis extracts (LLOS), were characterized using phenotyping and 16S rRNA identification. Seven strains were assigned to Streptococcus sp., most likely S. bovis, and were all degrading starch. One amylolytic lactate-utilizing strain of Selenomonas ruminantium was also found. Two strains of Clostridium bifermentans were identified and showed proteolytic activity. Two strains were assigned to Mitsuokella jalaludinii and were saccharolytic. One strain belonged to a Bacillus species and seven strains were affiliated with Escherichia coli. All of the 20 strains were able to use many sugars, but none of them were able to degrade the polysaccharides carboxymethylcellulose and xylans. The effect of three propolis extracts (LLOS B1, C1 and C3) was tested on the in vitro growth of four representative isolates of S. bovis, E. coli, M. jalaludinii and C. bifermentans. The growth of S. bovis, E. coli and M. jalaludinii was not affected by the three propolis extracts at 1 mg ml⁻¹. C. bifermentans growth was completely inhibited at this LLOS concentration, but this bacterium was partially resistant at lower concentrations. LLOS C3, with the lower concentration of phenolic compounds, was a little less inhibitory than B1 and C1 on this strain.