Severe Intestinal Inflammation in the Small Intestine of Mice Induced by Controllable Deletion of Claudin-7

• Published in: Digestive diseases and sciences Vol. 63; no. 5; pp. 1200 - 1209
• Main Authors: Li, Wen-Jing, Xu, Chang, Wang, Kun, Li, Teng-Yan, Wang, Xiao-Nan, Yang, Hui, Xing, Tiaosi, Li, Wen-Xia, Chen, Yan-Hua, Gao, Hong, Ding, Lei
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2018; Springer; Springer Nature B.V
• Subjects: Adenoma - chemically induced; Adenoma - diagnostic imaging; Adenoma - genetics; Adenoma - pathology; Analysis; Animals; Biochemistry; Blotting, Western; Claudins - genetics; Colorectal cancer; Disease Models, Animal; Enteritis - chemically induced; Enteritis - diagnostic imaging; Enteritis - genetics; Enteritis - pathology; Female; Gastroenterology; Gene Deletion; Genetic engineering; Hepatology; Hyperplasia; Hyperplasia - chemically induced; Hyperplasia - diagnostic imaging; Hyperplasia - genetics; Hyperplasia - pathology; Immunohistochemistry; Inflammation; Intestinal Neoplasms - chemically induced; Intestinal Neoplasms - diagnostic imaging; Intestinal Neoplasms - genetics; Intestinal Neoplasms - pathology; Intestine, Small - diagnostic imaging; Intestine, Small - pathology; Male; Medicine; Medicine & Public Health; Mice; Mice, Knockout - genetics; Microscopy, Electron, Scanning; Oncology; Original Article; Phenotype; Rodents; Tamoxifen; Tamoxifen - administration & dosage; Tight Junctions - pathology; Transplant Surgery; Inflammation; ICKO; Cre/Loxp; Cldn7
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-018-4973-z

Background As a potential tumor suppressor gene, Claudin-7 (Cldn7), which is a component of tight junctions, may play an important role in colorectal cancer occurrence and development. Aims To generate a knockout mouse model of inducible conditional Cldn7 in the intestine and analyze the phenotype of the mice after induction with tamoxifen. Methods We constructed Cldn7-flox transgenic mice and crossed them with Villin-CreERT2 mice. The Cldn7 inducible conditional knockout mice appeared normal and were well developed at birth. We induced Cldn7 gene deletion by injecting different dosages of tamoxifen into the mice and then conducted a further phenotypic analysis. Results After induction for 5 days in succession at a dose of 200 µl tamoxifen in sunflower oil at 10 mg/ml per mouse every time, the mice appeared dehydrated, had a lower temperature, and displayed inactivity or death. The results of hematoxylin–eosin staining showed that the intestines of the Cldn7 inducible conditional knockout mice had severe intestinal defects that included epithelial cell sloughing, necrosis, inflammation and hyperplasia. Owing to the death of ICKO mice, we adjusted the dose of tamoxifen to a dose of 100 µl in sunflower oil at 10 mg/ml per mouse (aged more than 8 weeks old) every 4 days. And we could induce atypical hyperplasia and adenoma in the intestine. Immunofluorescent staining indicated that the intestinal epithelial structure was destroyed. Electron microscopy experimental analysis indicated that the intercellular gap along the basolateral membrane of Cldn7 inducible conditional knockout mice in the intestine was increased and that contact between the cells and matrix was loosened. Conclusions We generated a model of intestinal Cldn7 inducible conditional knockout mice. Intestinal Cldn7 deletion induced by tamoxifen initiated inflammation and hyperplasia in mice.