The p53 protein is a suppressor of Atox1 copper chaperon in tumor cells under genotoxic effects

• Published in: PloS one Vol. 18; no. 12; p. e0295944
• Main Authors: Tsymbal, Sergey, Refeld, Aleksandr, Zatsepin, Viktor, Kuchur, Oleg
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.12.2023
• Subjects: Analysis; Antibodies; Antimitotic agents; Antineoplastic agents; Antineoplastic drugs; Antitumor activity; Apoptosis; Atox1 gene; Biology and life sciences; Cancer; Cancer therapies; Care and treatment; Cell cycle; Cell death; Cell signaling; Cell survival; Cellular signal transduction; Chemotherapy; Colorectal cancer; Complications and side effects; Copper; Copper - metabolism; Copper Transport Proteins; Cyclin-dependent kinases; Cytotoxicity; Damage; Deoxyribonucleic acid; DNA; DNA Damage; DNA repair; Drugs; Effectiveness; Enzymes; Genes; Genotoxicity; Heavy metals; Kinases; Medicine and Health Sciences; Metabolism; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; Molecular dynamics; p53 Protein; Patient outcomes; Physical Sciences; Physiological aspects; Proteins; Radiation; Radiation therapy; Survival; Target recognition; Therapeutic applications; Transcription factors; Transition metals; Tumor cells; Tumor proteins; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Tumors; Russia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0295944

The p53 protein is crucial for regulating cell survival and apoptosis in response to DNA damage. However, its influence on therapy effectiveness is controversial: when DNA damage is high p53 directs cells toward apoptosis, while under moderate genotoxic stress it saves the cells from death and promote DNA repair. Furthermore, these processes are influenced by the metabolism of transition metals, particularly copper since they serve as cofactors for critical enzymes. The metallochaperone Atox1 is under intensive study in this context because it serves as transcription factor allegedly mediating described effects of copper. Investigating the interaction between p53 and Atox1 could provide insights into tumor cell survival and potential therapeutic applications in oncology. This study explores the relationship between p53 and Atox1 in HCT116 and A549 cell lines with wild type and knockout TP53. The study found an inverse correlation between Atox1 and p53 at the transcriptional and translational levels in response to genotoxic stress. Atox1 expression decreased with increased p53 activity, while cells with inactive p53 had significantly higher levels of Atox1. Suppression of both genes increased apoptosis, while suppression of the ATOX1 gene prevented apoptosis even under the treatment with chemotherapeutic drugs. The findings suggest that Atox1 may act as one of key elements in promotion of cell cycle under DNA-damaging conditions, while p53 works as an antagonist by inhibiting Atox1. Understanding of this relationship could help identify potential targets in cell signaling pathways to enhance the effectiveness of combined antitumor therapy, especially in tumors with mutant or inactive p53.