Shoot control of root development and nodulation is mediated by a receptor-like kinase

• Published in: Nature (London) Vol. 420; no. 6914; pp. 422 - 426
• Main Authors: Stougaard, Jens, Krusell, Lene, Madsen, Lene H, Sato, Shusei, Aubert, Grégoire, Genua, Aratz, Szczyglowski, Krzysztof, Duc, Gérard, Kaneko, Takakazu, Tabata, Satoshi, de Bruijn, Frans, Pajuelo, Eloisa, Sandal, Niels
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 28.11.2002; Nature Publishing Group
• Subjects: Agronomy. Soil science and plant productions; Alleles; Amino Acid Sequence; Biological and medical sciences; Cellular Biology; Cloning; Cloning, Molecular; Economic plant physiology; Enzymes; Feedback, Physiological; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Genes, Plant - genetics; Legumes; Life Sciences; Lotus - enzymology; Lotus - genetics; Lotus - metabolism; Lotus - microbiology; Molecular Sequence Data; Mutation; Mutation - genetics; Nitrogen fixation; Nitrogen Fixation - genetics; Parasitism and symbiosis; Pisum sativum - enzymology; Pisum sativum - genetics; Plant growth; Plant physiology and development; Plant Roots - enzymology; Plant Roots - genetics; Plant Roots - metabolism; Plant Roots - microbiology; Plant Shoots - enzymology; Plant Shoots - genetics; Plant Shoots - metabolism; Plant Shoots - microbiology; Plant tissues; Protein Serine-Threonine Kinases - chemistry; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Protein Structure, Tertiary; Rhizobium - physiology; Root development; Roots; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiosis - genetics; Symbiosis; Enzyme; Transferases; Oligosaccharide; Nodulation; Feedback regulation; Plant nodule; Organogenesis; Regulation(control); Leguminosae; Gene; Protein kinase; Dicotyledones; Angiospermae; Spermatophyta; Mutation; Plant microorganism relation; Biological receptor; CONTROLE DE MALADIES
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/nature01207

In legumes, root nodule organogenesis is activated in response to morphogenic lipochitin oligosaccharides that are synthesized by bacteria, commonly known as rhizobia. Successful symbiotic interaction results in the formation of highly specialized organs called root nodules, which provide a unique environment for symbiotic nitrogen fixation. In wild-type plants the number of nodules is regulated by a signalling mechanism integrating environmental and developmental cues to arrest most rhizobial infections within the susceptible zone of the root. Furthermore, a feedback mechanism controls the temporal and spatial susceptibility to infection of the root system. This mechanism is referred to as autoregulation of nodulation, as earlier nodulation events inhibit nodulation of younger root tissues. Lotus japonicus plants homozygous for a mutation in the hypernodulation aberrant root (har1) locus escape this regulation and form an excessive number of nodules. Here we report the molecular cloning and expression analysis of the HAR1 gene and the pea orthologue, Pisum sativum, SYM29. HAR1 encodes a putative serine/threonine receptor kinase, which is required for shoot-controlled regulation of root growth, nodule number, and for nitrate sensitivity of symbiotic development.