Metabolomic profiling of urinary changes in mice with monosodium glutamate-induced obesity

• Published in: Analytical and bioanalytical chemistry Vol. 408; no. 2; pp. 567 - 578
• Main Authors: Pelantová, Helena, Bártová, Simona, Anýž, Jiří, Holubová, Martina, Železná, Blanka, Maletínská, Lenka, Novák, Daniel, Lacinová, Zdena, Šulc, Miroslav, Haluzík, Martin, Kuzma, Marek
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2016; Springer; Springer Nature B.V
• Subjects: acetates; adiponectin; Age; allantoin; Analytical Chemistry; animal models; Animals; Biochemistry; Blood Glucose - metabolism; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; citrates; Complications and side effects; creatine; Diabetes; Enzymes; excretion; Food; Food Science; Gene expression; Humans; Hypothalamus; Insulin; Insulin - metabolism; Insulin resistance; Laboratory animals; Laboratory Medicine; Lipid Metabolism; liver; Liver - metabolism; Male; messenger RNA; Metabolism; Metabolomics; methylamine; Mice; Monitoring/Environmental Analysis; Monosodium glutamate; nicotinamide; nuclear magnetic resonance spectroscopy; Obesity; Obesity - etiology; Obesity - genetics; Obesity - metabolism; Obesity - urine; Peroxisome Proliferator-Activated Receptors - genetics; Peroxisome Proliferator-Activated Receptors - metabolism; Physiological aspects; Polyamines; proteins; Research Paper; Sodium Glutamate - adverse effects; succinic acid; urinalysis; Urine; Urine - chemistry; Czech Republic; Massachusetts; Urine; Metabolomics; Monosodium glutamate (MSG) induced obesity; NMR; Mouse model; Diabetes
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-015-9133-0

Obesity with related complications represents a widespread health problem. The etiopathogenesis of obesity is often studied using numerous rodent models. The mouse model of monosodium glutamate (MSG)-induced obesity was exploited as a model of obesity combined with insulin resistance. The aim of this work was to characterize the metabolic status of MSG mice by NMR-based metabolomics in combination with relevant biochemical and hormonal parameters. NMR analysis of urine at 2, 6, and 9 months revealed altered metabolism of nicotinamide and polyamines, attenuated excretion of major urinary proteins, increased levels of phenylacetylglycine and allantoin, and decreased concentrations of methylamine in urine of MSG-treated mice. Altered levels of creatine, citrate, succinate, and acetate were observed at 2 months of age and approached the values of control mice with aging. The development of obesity and insulin resistance in 6-month-old MSG mice was also accompanied by decreased mRNA expressions of adiponectin, lipogenetic and lipolytic enzymes and peroxisome proliferator-activated receptor-gamma in fat while mRNA expressions of lipogenetic enzymes in the liver were enhanced. At the age of 9 months, biochemical parameters of MSG mice were normalized to the values of the controls. This fact pointed to a limited predictive value of biochemical data up to age of 6 months as NMR metabolomics confirmed altered urine metabolic composition even at 9 months.