Prognostic value of microRNA-126 and CRK expression in gastric cancer

• Published in: OncoTargets and therapy Vol. 9; pp. 6127 - 6135
• Main Authors: Yue, Shun, Shi, Huichang, Han, Jun, Zhang, Tiecheng, Zhu, Weiguo, Zhang, Dahong
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2016; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Care and treatment; Cell cycle; Cell growth; Colorectal cancer; Development and progression; Gastric cancer; Gene expression; Genes; Genetic aspects; Growth factors; Health aspects; Hospitals; Kinases; Lung cancer; Medical prognosis; MicroRNA; MicroRNAs; Oncogenes; Oncology; Original Research; Polymerase chain reaction; Prognosis; Sarcoma; Software; Statistical analysis; Stomach cancer; United States > US; China; real-time quantitative polymerase chain reaction; miR-126; prognosis; v-crk sarcoma virus CT10 oncogene homologue
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S87778

MicroRNA (miR)-126, acting as a tumor suppressor, has been reported to inhibit the invasion of gastric cancer cells in part by targeting v-crk sarcoma virus CT10 oncogene homologue (CRK). The aim of this study was to investigate the clinical significance of miR-126/CRK axis in gastric cancer. miR-126 and CRK mRNA expression levels were detected by real-time quantitative reverse transcription polymerase chain reaction in 220 self-pairs of gastric cancer and adjacent noncancerous tissues. Expression levels of miR-126 and CRK mRNA in gastric cancer tissues were, respectively, lower and higher than those in adjacent noncancerous tissues (both <0.001). Low miR-126 expression and high CRK expression, alone or in combination, were all significantly associated with positive lymph node and distant metastases and advanced TNM stage of human gastric cancer (all <0.05). We also found that the overall survival rates of the patients with low miR-126 expression and high CRK expression were, respectively, shorter than those with high miR-126 expression and low CRK expression. Interestingly, miR-126-low/CRK-high expression was associated with a significantly worse overall survival of all miR-126/CRK groups ( <0.001). Moreover, multivariate analysis identified miR-126 and/or CRK expression as independent prognostic factors for patients with gastric cancer. Notably, the prognostic relevance of miR-126 and/or CRK expression was more obvious in the subgroup of patients with TNM stage IV. Dysregulation of miR-126/CRK axis may promote the malignant progression of human gastric cancer. miR-126 and CRK combined expression may serve as an independent predictor of overall survival in patients with advanced gastric cancer.