Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD

• Published in: Applied and Environmental Microbiology Vol. 60; no. 10; pp. 3870 - 3873
• Main Authors: Christensen, D.P, Hutkins, R.W
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1994
• Subjects: Bacteria; BACTERIOCINAS; BACTERIOCINE; Bacteriocins - pharmacology; Biological and medical sciences; Biological Transport, Active - drug effects; Biology; Cell physiology; Deoxyglucose - metabolism; Food contamination & poisoning; Fundamental and applied biological sciences. Psychology; GLUCOSA; GLUCOSE; Glucose - metabolism; INHIBICION; INHIBITION; LISTERIA MONOCYTOGENES; Listeria monocytogenes - drug effects; Listeria monocytogenes - metabolism; Membrane and intracellular transports; Molecular and cellular biology; Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism; Phosphorylation; PROPIEDADES ANTIMICROBIANAS; PROPRIETE ANTIMICROBIENNE; Protons; TRANSPORT DES SUBSTANCES NUTRITIVES; TRANSPORTE DE NUTRIENTES; Uncoupling Agents - pharmacology; Culture medium; Microorganism growth; Phosphorylation; Enzyme; Bacteriocin; Listeria monocytogenes; Glucose; Permeability; Motive force; Isomerases; Enzymatic activity; Membrane transport; Intramolecular transferases; Microorganism culture; Bacteria; Inhibition; ATP; Phosphotransferases
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.60.10.3870-3873.1994

Glucose uptake by Listeria monocytogenes Scott A was inhibited by the bacteriocin pediocin JD and by the protonophore carbonyl cyanide m-chlorophenyhydrazone. Experiments with monensin, nigericin, chlorhexidine diacetate, dinitrophenol, and gramicidin, however, showed that glucose uptake could occur in the absence of a proton motive force. L. monocytogenes cell extracts phosphorylated glucose when phosphoenolpyruvate (PEP) was present in the assay mixture, and whole cells incubated with 2-deoxyglucose accumulated 2-deoxyglucose-6-phosphate, indicating the presence of a PEP-dependent phosphotransferase system in this organism. Glucose phosphorylation also occurred when ATP was present, suggesting that a proton motive force-mediated glucose transport system may also be present. We conclude that L. monocytogenes Scott A accumulates glucose by phosphotransferase and proton motive force-mediated systems, both of which are sensitive to pediocin JD