Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense

• Published in: AMB Express Vol. 9; no. 1; pp. 11 - 10
• Main Authors: Liu, Dongxin, He, Wencong, Jiang, Mingxia, Zhao, Bing, Ou, Xichao, Liu, Chunfa, Xia, Hui, Zhou, Yang, Wang, Shengfen, Song, Yuanyuan, Zheng, Yang, Chen, Qian, Fan, Jiale, He, Guangxue, Zhao, Yanlin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 23.01.2019; Springer Nature B.V; SpringerOpen
• Subjects: Antimicrobial agents; Biomedical and Life Sciences; Biotechnology; Chronic infection; Clinical outcomes; Cross-reaction; Design; Diagnosis; Drug resistance; Drug susceptibility testing; erm gene; Infections; Laboratories; LAMP; Life Sciences; Lung diseases; Microbial Genetics and Genomics; Microbiology; Mycobacterium; Mycobacterium abscessus; Mycobacterium massiliense; Original; Original Article; Pathogens; Surgical site infections; Wound infection; LAMP; Diagnosis; Drug susceptibility testing; gene; Mycobacterium massiliense; erm gene; Mycobacterium abscessus
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-019-0734-4

Mycobacterium abscessus ( M. abscessus ) and Mycobacterium massiliense ( M. massiliense ) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense , via specific erm (41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense , no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC ( M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections.