Possible Mechanisms of Sudden Death and Hemoconcentration Induced by Endothelin-1 and Big Endothelin-1 in Mice

• Published in: Biological & pharmaceutical bulletin Vol. 17; no. 5; pp. 645 - 650
• Main Authors: OKUMURA, Hiroshi, ASHIZAWA, Naoki, AOTSUKA, Tomoji, ASAKURA, Rieko, KOBAYASHI, Fujio, MATSUURA, Akihiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1994; Maruzen; Japan Science and Technology Agency
• Subjects: Adrenergic alpha-Antagonists - pharmacology; Adrenergic beta-Agonists - pharmacology; Adrenergic beta-Antagonists - pharmacology; Animals; Aorta - enzymology; Aspartic Acid Endopeptidases - antagonists & inhibitors; big endothelin-1; Biological and medical sciences; Calcium Channel Blockers - pharmacology; calcium-channel blocker; Cattle; Death, Sudden - etiology; Drug toxicity and drugs side effects treatment; Endothelin-1; Endothelin-Converting Enzymes; Endothelins - antagonists & inhibitors; Endothelins - toxicity; Hematocrit; hemoconcentration; Humans; Male; Medical sciences; Metalloendopeptidases - antagonists & inhibitors; Mice; Mice, Inbred ICR; mouse; Pharmacology. Drug treatments; Protease Inhibitors - pharmacology; Protein Precursors - antagonists & inhibitors; Protein Precursors - toxicity; sudden death; Toxicity: cardiovascular system; Vertebrata; Mammalia; Intravenous administration; Mouse; Toxicity; Animal; Sudden death; Rodentia; Vasoconstrictor agent; Endothelin 1; Mechanism of action
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.17.645

We investigated the profiles of sudden death and hemoconcentration induced by endothelin-1 (ET-1) and big endothelin-1 (big ET-1) in mice using various compounds as pharmacological tools. In ET-1-induced sudden death (5 nmol/kg, i.v.), pretreatment with the Ca2+-channel blockers, diltiazem, nifedipine or verapamil at a dose of 2 mg/kg, i.v. significantly inhibited the mortality and prolonged the latency to death. These Ca2+-channel blockers, however, failed to inhibit the rise in hematocrit (Ht), namely hemoconcentration, induced by ET-1 (2.5 nmol/kg). A beta-adrenoceptor agonist, isoproterenol (1 mg/kg) tended to prolong the latency, whereas, a beta-adrenoceptor blocker, propranolol (2 mg/kg), and an alpha-and beta-adrenoceptor blocker, labetalol (5 mg/kg), aggravated the sudden death. Esculetin (10 mg/kg) and fenbufen (10 mg/kg), which are enzyme inhibitors in the arachidonate cascade, prevented only the hemoconcentration. Anti-arrythmic drugs, lidocaine (1 mg/kg) and disopyramide (20 mg/kg) did not improve any parameters. Big ET-1 also caused sudden death (20 and 25 nmol/kg, i.v.) and hemoconcentration (10 nmol/kg, i.v.). Of several proteinase inhibitors, only a metalloproteinase inhibitor, phosphoramidon (2 mg/kg i.v.), prevented the sudden death and the hemoconcentration induced by big ET-1 but not by ET-1. Ca2+-channel blockers exerted their protective effects only when a lower dose of big ET-1 was employed. These results indicate that the sudden death caused by both peptides is mainly due to myocardial ischemia and respiratory disorder, and that hemoconcentration caused by them is due not to their vasoconstrictor action but to their effects on the vascular permeability via secondary endogenous factors.