Beta-Endorphin Binding Activity of SP-40, 40

• Published in: Biological & pharmaceutical bulletin Vol. 16; no. 3; pp. 228 - 231
• Main Authors: CHOI-MIURA, Nam-Ho, NAKANO, Yasuko, TOBE, Takashi, MAZDA, Toshio, TOMITA, Motowo
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1993; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; Autoradiography; beta-endorphin; beta-Endorphin - metabolism; Biological and medical sciences; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Clusterin; Complement Membrane Attack Complex - metabolism; coplement; Cross-Linking Reagents - pharmacology; Extracellular Matrix Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Glycoproteins - metabolism; Glycoproteins - pharmacology; Humans; In Vitro Techniques; Iodine Radioisotopes; Molecular Chaperones; Nerve Tissue Proteins - metabolism; Nerve Tissue Proteins - pharmacology; Protein Binding; Rats; Receptors, Opioid - drug effects; Receptors, Opioid - metabolism; SC5b-9; SMAC; SP-40, 40; Vertebrates: nervous system and sense organs; Vitronectin; Rat; Rodentia; Central nervous system; Peptide hormone; Vitronectin; Complement; Glycoproteins; β-Endorphin; Neuropeptide; C terminal-Sequence; Vertebrata; Biological fixation; Mammalia; Animal; Brain (vertebrata)
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.16.228

SP-40, 40 bound to β-endorphin via C-terminal non-opioid portion of β-endorphin as well as S-protein (vitronectin) bound. Beta-endorphin bound mainly to SP-40, 40, but not to S-protein, in the soluble membrane attack complex (SMAC, SC5b-9) of complement, because the results of autoradiography of the cross-linking experiment of SMAC with [125I]β-endorphin revealed only a typical band of SP-40, 40. The binding of SP-40, 40 to β-endorphin inhibited the binding of β-endorphin to its receptor of rat brain; thus SP-40, 40 might inhibit the biological action of β-endorphin.