A cross-reactive neutralizing monoclonal antibody protects mice from H5N1 and pandemic (H1N1) 2009 virus infection

• Published in: Antiviral research Vol. 88; no. 3; pp. 249 - 255
• Main Authors: Sakabe, Saori, Iwatsuki-Horimoto, Kiyoko, Horimoto, Taisuke, Nidom, Chairul A., Quynh Le, Mai thi, Takano, Ryo, Kubota-Koketsu, Ritsuko, Okuno, Yoshinobu, Ozawa, Makoto, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.12.2010; Elsevier
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; Antibodies, Monoclonal - therapeutic use; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - isolation & purification; Antibodies, Neutralizing - therapeutic use; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; Antibodies, Viral - isolation & purification; Antibodies, Viral - therapeutic use; Biological and medical sciences; Cross Reactions; Drug Administration Routes; Epitopes - chemistry; Epitopes - immunology; Female; Fundamental and applied biological sciences. Psychology; H5N1; Hemagglutinins - chemistry; Hemagglutinins - immunology; Human viral diseases; Humans; Immunotherapy - methods; Infectious diseases; Influenza A Virus, H1N1 Subtype - chemistry; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H5N1 Subtype - chemistry; Influenza A Virus, H5N1 Subtype - immunology; Influenza, Human - immunology; Influenza, Human - prevention & control; Influenza, Human - virology; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Monoclonal antibody Immunotherapy; Pandemic (H1N1) 2009; Pandemics; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral diseases; Viral diseases of the respiratory system and ent viral diseases; Virology; Pandemic (H1N1) 2009; H5N1; Monoclonal antibody Immunotherapy; Intraperitoneal administration; Orthomyxoviridae; Monoclonal antibody; HSN1; Prevention; Avian influenzavirus; Immunotherapy; Cross reaction; Respiratory disease; H1N1 influenza; Rodentia; Intranasal administration; Neutralizing antibody; Infection; Virus; Influenzavirus A(H5N1); Vertebrata; Influenzavirus A; Mammalia; Treatment; Mouse; Animal; Viral disease; Neutralization
• ISSN: 0166-3542; 1872-9096; 1872-9096
• DOI: 10.1016/j.antiviral.2010.09.007

A novel influenza (H1N1) virus caused an influenza pandemic in 2009, while highly pathogenic H5N1 avian influenza viruses have continued to infect humans since 1997. Influenza, therefore, remains a serious health threat. Currently, neuraminidase (NA) inhibitors are the mainstay for influenza therapy; however, drug-resistant mutants of seasonal H1N1 and H5N1 viruses have emerged highlighting the need for alternative therapeutic approaches. One such approach is antibody immunotherapy. Here, we show that the monoclonal antibody C179, which recognizes a neutralizing epitope common among H1, H2, H5, and H6 hemagglutinins (HAs), protected mice from a lethal challenge with various H5N1 and pandemic (H1N1) 2009 viruses when administered either intraperitoneally or intranasally. The protective efficacy of intranasally inoculated C179 was comparable to that of intraperitoneal administration. Our results suggest that direct administration of this anti-influenza antibody to viral replication sites is an effective strategy for prophylaxis and therapy.