Derivation of DNA probes for enumeration of a specific strain of Lactobacillus acidophilus in piglet digestive tract samples

• Published in: Applied and Environmental Microbiology Vol. 59; no. 11; pp. 3871 - 3877
• Main Authors: Rodtong, S, Dobbinson, S, Thode-Andersen, S, McConnell, M.A, Tannock, G.W
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.1993
• Subjects: ADN; ANALISIS BIOLOGICO; ANALYSE BIOLOGIQUE; Animal, plant and microbial ecology; Animals; APPAREIL DIGESTIF; BACTERIA; Bacteriology; Biological and medical sciences; CONTEO DE CELULAS; Deoxyribonucleic acid; Digestive system; Digestive System - microbiology; DNA; DNA Probes; DNA, Bacterial - genetics; FLORA MICROBIANA; FLORE MICROBIENNE; Fundamental and applied biological sciences. Psychology; HIBRIDACION DE ADN; Hogs; HYBRIDATION D'ADN; INTESTIN; INTESTINOS; LACTOBACILLUS ACIDOPHILUS; Lactobacillus acidophilus - genetics; Lactobacillus acidophilus - isolation & purification; LECHON; Microbial ecology; Molecular Probe Techniques - statistics & numerical data; Normal microflora of man and animals. Rumen; Nucleic Acid Hybridization; NUMERATION CELLULAIRE; Plasmids; PORCELET; Sensitivity and Specificity; SISTEMA DIGESTIVO; Swine - microbiology; Molecular hybridization; Lactobacillus acidophilus; Young animal; Enumeration(counting); Pig; Plasmid; Vertebrata; Specificity; Mammalia; Bacteria; Lactobacillaceae; Colony; Chromosome DNA; Associated microflora; Molecular probe; Artiodactyla; Digestive tract; Ungulata; Colonization
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.59.11.3871-3877.1993

Four DNA probes were derived that hybridized specifically to DNA from Lactobacillus acidophilus 0. The probes were constructed by randomly cloning lactobacillus DNA in plasmid vector pBR322. Two of the probes (pSR1 and pSR2) were composed of vector and plasmid DNA inserts (3.6 and 1.6 kb, respectively); the others (pSR3 and pSR4) were composed of vector and chromosomally derived inserts (6.9 and 1.4 kb, respectively). The probes were used to enumerate, by colony hybridization, strain O in digestive tract samples collected from piglets inoculated 24 hours previously with a culture of the strain. The probes did not hybridize to DNA from lactobacilli inhabiting the digestive tract of uninoculated piglets. Strain O made up about 10% of the total lactobacillus population of the pars esophageal and about 20% of the population in other digestive tract samples