Effects of Cyclophosphamide on the Expression and Induction of Delayed Hypersensitivity

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 22; no. 11; pp. 719 - 730
• Main Authors: Himeno, Kunisuke, Nomoto, Kikuo, Takeya, Kenji
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.01.1978; Center For Academic Publications Japan
• Subjects: Animals; Basophils - pathology; Cyclophosphamide - pharmacology; Female; Freund's Adjuvant; gamma-Globulins - immunology; Guinea Pigs; Hypersensitivity, Delayed - etiology; Hypersensitivity, Delayed - immunology; Immunization; Leukocyte Count; Macrophage Migration-Inhibitory Factors - biosynthesis; Skin Tests
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1978.tb00425.x

Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reactions from negative to positive at late intervals after immunization with BGG in IFA. Such a treatment augmented erythematous delayed reactions in animals immunized with BGG in CFA, but abolished induration at the reaction sites. CY-treatment before elicitation or immunization reduced the numbers of basophils at the reaction sites, although erythematous delayed reactions were augmented. Effector T cells responsible for delayed erythematous reaction without induration appear to persist for a long period of time after immunization in the presence of antibody production or tuberculin hypersensitivity and the expression of their function may be inhibited by suppressive mechanisms.