胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响

目的观察胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响。方法选取行体外受精治疗的受试者24对,获得168个受精胚胎,随机分为A、B、C、D组,每组42个胚胎。四组胚胎均在CO_2培养箱中,在37℃、6%CO_2条件下连续培养6 d并进行观察。采用Primo vision胚胎监测仪观察各组胚胎的发育过程,A、B、C、D组的拍摄时间间隔分别为5、10、15、20 min。胚胎监测结束后,找到每个胚胎对应的监测照片和模拟视频。记录胚胎多细胞发育所需时间,观察完成受精操作60 h时胚胎发育情况,计算各组形成优质胚胎和囊胚的比例,记录各组胚胎初次分裂的卵裂模式。结果 A组胚胎发育到5细胞所需时间长于...

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Bibliographic Details
Published in山东医药 Vol. 57; no. 3; pp. 5 - 8
Main Author 柴蓓蓓 于成和 李正伟 许益娟 何培 张若鹏
Format Magazine Article
LanguageChinese
Published 大理大学临床医学院,云南大理,671000%大理大学第一附属医院 2017
Subjects
体外受精
多细胞胚胎
延时摄像技术
胚胎发育
胚胎监测
辅助生殖技术
体外受精
assisted reproductive technology
胚胎发育
embryonic development
延时摄像技术
多细胞胚胎
embryo monitoring
in vitro fertilization
胚胎监测
辅助生殖技术
time-lapse camera technology
multicellular embryo
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2017.03.002

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Summary:目的观察胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响。方法选取行体外受精治疗的受试者24对,获得168个受精胚胎,随机分为A、B、C、D组,每组42个胚胎。四组胚胎均在CO_2培养箱中,在37℃、6%CO_2条件下连续培养6 d并进行观察。采用Primo vision胚胎监测仪观察各组胚胎的发育过程,A、B、C、D组的拍摄时间间隔分别为5、10、15、20 min。胚胎监测结束后,找到每个胚胎对应的监测照片和模拟视频。记录胚胎多细胞发育所需时间,观察完成受精操作60 h时胚胎发育情况,计算各组形成优质胚胎和囊胚的比例,记录各组胚胎初次分裂的卵裂模式。结果 A组胚胎发育到5细胞所需时间长于D组,A、B组发育到6、7、8细胞所需时间长于D组,A组发育到6、7、8细胞所需时间长于C组（P均〈0.05）。完成受精操作60 h时A组胚胎仍处在6细胞阶段,B组胚胎尚未发育到7细胞阶段,C组胚胎已分裂至7细胞阶段,D组为8细胞胚胎。C、D组形成优质胚胎、囊胚的比例均高于A组,C、D组形成囊胚的比例高于B组（P均〈0.05）。C、D组初次正常卵裂胚胎比例高于A组,D组正常卵裂胚胎比例高于B组,C组非轴性卵裂、非二倍性卵裂胚胎比例低于A组,C组不均一性卵裂胚胎比例高于A组,D组非轴性卵裂胚胎比例低于A组和B组（P均〈0.05）。结论胚胎监测仪延时摄像间隔时间对胚胎发育存在一定影响,采用15 min和20 min的拍摄间隔较5 min、10 min对胚胎发育影响更小。
Bibliography:embryo monitoring; time-lapse camera technology; assisted reproductive technology; in vitro fertilization; embryonic development; multicellular embryo
Objective To investigate the effect of time-lapse interval of embryo monitoring on human embryonic development after in vitro fertilization. Methods Totally 168 fertilized embryos were obtained from 24 couples who received the treatment of in vitro fertilization（IVF） and then were randomly divided into 4 groups：groups A,B,C and D,42 embryos in each group. The embryos in the four groups were continuously monitored for 6 days in a CO2 incubator at 37℃ with 6%CO2. Each group＇s embryo development was observed by Primo vision embryo monitor and the time-lapse interval in groups A,B,C and D was 5,10,15 and 20 min,respectively. After the embryo monitoring,we found the corresponding monitoring photos and analog video for each embryo. We recorded the time of each embryo＇ s multicellular development and the embryo development at 60 h after in vitro fertilization. The prop
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2017.03.002