HBV感染对肝脏自然杀伤细胞和固有淋巴样细胞22的影响

目的探讨HBV感染对肝脏自然杀伤细胞（NK细胞）和固有淋巴样细胞（ILC）22的影响,为阐明HBV感染诱导机体固有免疫应答的机制提供理论和实验依据。方法选取6~8周龄雄性BALB/c小鼠10只,随机分为实验组和对照组,每组各5只。实验组小鼠经尾静脉高压注射含10μg HBV全基因组质粒的生理盐水（体积相当于9%小鼠体质量）;对照组小鼠仅注射生理盐水。4 d后处死小鼠,分离肝脏内淋巴细胞,应用流式细胞术检测肝脏内淋巴细胞中NK细胞亚群和ILC22细胞亚群的比例。组间比较采用t检验。结果高压尾静脉注射HBV全基因组质粒可诱导小鼠产生高水平的HBs Ag和HBe Ag,伴有血清ALT水平升高。实验组...

Full description

Saved in:
Bibliographic Details
Published in临床肝胆病杂志 Vol. 32; no. 10; pp. 1883 - 1887
Main Author 彭梅娟 杨晓飞 魏欣 连建奇 张野
Format Journal Article
LanguageChinese
Published 第四军医大学唐都医院全军感染病诊疗中心,西安,710038 2016
Subjects
免疫,先天
杀伤细胞,天然
淋巴细胞
肝炎病毒,乙型
lymphocytes
immunity,innate
hepatitis B virus
killer cells,natural
杀伤细胞,天然
淋巴细胞
免疫,先天
肝炎病毒,乙型
Online AccessGet full text
ISSN1001-5256
DOI10.3969/j.issn.1001-5256.2016.10.012

Cover

More Information
Summary:目的探讨HBV感染对肝脏自然杀伤细胞（NK细胞）和固有淋巴样细胞（ILC）22的影响,为阐明HBV感染诱导机体固有免疫应答的机制提供理论和实验依据。方法选取6~8周龄雄性BALB/c小鼠10只,随机分为实验组和对照组,每组各5只。实验组小鼠经尾静脉高压注射含10μg HBV全基因组质粒的生理盐水（体积相当于9%小鼠体质量）;对照组小鼠仅注射生理盐水。4 d后处死小鼠,分离肝脏内淋巴细胞,应用流式细胞术检测肝脏内淋巴细胞中NK细胞亚群和ILC22细胞亚群的比例。组间比较采用t检验。结果高压尾静脉注射HBV全基因组质粒可诱导小鼠产生高水平的HBs Ag和HBe Ag,伴有血清ALT水平升高。实验组小鼠肝脏内NK细胞的比例较对照组小鼠显著升高[（25.90±4.92）%vs（12.98±2.13）%,t=3.811,P=0.003],但CD127＋NK细胞和CD127-NK细胞亚群在NK细胞中所占比例在2组之间差异均无统计学意义（P值均〉0.05）。实验组小鼠肝脏内NKp46＋ILC22细胞亚群比例较对照组明显升高[（36.05±6.85）%vs（10.22±3.54）%,t=7.372,P〈0.001],但NKp46-ILC22细胞亚群的比例在2组之间差异无统计学意义（P〉0.05）。结论 HBV感染可诱导肝脏内NK细胞和NKp46＋ILC22水平升高,从而促进肝脏固有免疫应答。
Bibliography:hepatitis B virus; killer cells; natural; lymphocytes; immunity; innate
Objective To investigate the influence of hepatitis B virus（ HBV） infection on intrahepatic natural killer（ NK） cells and innate lymphoid cell 22（ ILC22）,and to provide theoretical and experimental bases for clarifying mechanisms of HBV infection in inducing innate immune response. Methods A total of 10 male BALC / c aged 6-8 weeks were divided into experimental group and control group,with 5 mice in each group. The mice in the experimental group were treated with the hydrodynamic injection of normal saline containing 10μg plasmids of complete HBV genome（ the volume equaled to 9% of the body weight of the mouse） via the caudal vein,and those in the control group were only treated with normal saline. The mice were scarified 4 days later,and intrahepatic lymphocytes（ IHLs） were isolated.Flow cytometry was used to determine the proportions of NK cells and ILC22 subset in IHLs,and the t-test was used for comparison between groups. Results Hydr
ISSN:1001-5256
DOI:10.3969/j.issn.1001-5256.2016.10.012