Alkaline and Halophilic Protease Production by Bacillus luteus H11 and its Potential Industrial Applications

• Published in: Food technology and biotechnology Vol. 56; no. 4; pp. 553 - 561
• Main Authors: Kalwasińska, Agnieszka, Jankiewicz, Urszula, Felföldi, Tamás, Burkowska-But, Aleksandra, Swiontek Brzezinska, Maria
• Format: Journal Article
• Language: English
• Published: Croatia Sveuciliste U Zagrebu 2018; Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet; University of Zagreb Faculty of Food Technology and Biotechnology
• Subjects: Alkaline protease; Alkalinity; alkalohalophiles; Azocasein; Bacillus; Bacillus luteus; Bacteria; Bioremediation; Chitinase; Detergents; Environmental management; Enzymes; Food industry; Glucose; Industrial applications; Microbiological synthesis; Microorganisms; Organic solvents; Original Scientific Papers; Oxidants; Oxidizing agents; pH effects; Pharmaceutical industry; Phenylalanine; Physiological aspects; Production processes; Protease; Proteases; proteolytic bacteria; Salinity; Salinity tolerance; Serine; serine endoprotease; Sodium chloride; Solvents; Substrates; Subtilisin; subtilisins; Surface active agents; Hungary; Poland; United States > US; Germany; subtilisins; alkalohalophiles; proteolytic bacteria; serine endoprotease; Bacillus
• ISSN: 1330-9862; 1334-2606
• DOI: 10.17113/ftb.56.04.18.5553

This paper presents the results of the study on the production of protease by H11 isolated from an alkaline soda lime. H11 was identified as an alkalohalophilic bacterium, and its extracellular serine endoprotease also showed an extreme alkali- and halotolerance. It was remarkably stable in the presence of NaCl up to 5 M. The enzyme was active in a broad range of pH values and temperatures, with an optimum pH of 10.5 and a temperature of 45 °C. It had a molecular mass of about 37 kDa and showed activity against azocasein and a synthetic substrate for the subtilisin-like protease, N-succinyl-l-phenylalanine- -nitroanilide. The halo-alkaline protease produced by H11 seems to be significant from an industrial perspective because of its tolerance towards high salinity and alkalinity as well as its stability against some organic solvents, surfactants and oxidants. These properties make the protease suitable for applications in food, detergent and pharmaceutical industries, and also in environmental bioremediation.