Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli

Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication....

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Bibliographic Details
Published inScientific reports Vol. 11; no. 1; pp. 474 - 8
Main Authors Helgesen, Emily, Sætre, Frank, Skarstad, Kirsten
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 12.01.2021
Nature Publishing Group
Nature Portfolio
Subjects
631/337/151/2353
631/337/641/2002
Cell cycle
Chromosomes
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA topoisomerase IV
E coli
Humanities and Social Sciences
multidisciplinary
Replication
Science
Science (multidisciplinary)
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Summary:Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.
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HSØ/2012062
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-80043-4