Rapid and convenient detection of urinary equol by thin-layer chromatography
Equol, a metabolite of daidzein for some intestinal microflora, is known to retain highly estrogenic activity and is of wide interest in relation to human health. However, not all humans can produce equol. In this study, detection of urinary equol using thin-layer chromatography (TLC) was performed...
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Published in | Journal of Nutritional Science and Vitaminology Vol. 53; no. 1; pp. 43 - 47 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Tokyo
Center for Academic Publications Japan
01.02.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Equol, a metabolite of daidzein for some intestinal microflora, is known to retain highly estrogenic activity and is of wide interest in relation to human health. However, not all humans can produce equol. In this study, detection of urinary equol using thin-layer chromatography (TLC) was performed to distinguish between equol producers and non-producers. After 36 h of soy food intake, urine, collected from 7 volunteers, was hydrolyzed, purified by reverse phase silica gel column and applied to normal phase TLC. Consequently, equol was clearly separated from the urine samples and discriminated the equol producers in this system. The detection limit of equol was at least 20 ng. |
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Bibliography: | U40 Q04 2007007307 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0301-4800 1881-7742 |
DOI: | 10.3177/jnsv.53.43 |