Synthesis of CdSe Quantum Dots Using Fusarium oxysporum
CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using . The cadmium and selenium concentration, pH, and temperature for the culture of ( ) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial c...
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Published in | Materials Vol. 9; no. 10; p. 855 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
20.10.2016
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using
. The cadmium and selenium concentration, pH, and temperature for the culture of
(
) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial cells of
were observed by transmission electron microscopy. Ultra-thin sections of
showed that the CdSe quantum dots were precipitated in the intracellular space, indicating that cadmium and selenium ions were incorporated into the cell and that the quantum dots were synthesized with intracellular metabolites. To reveal differences in
metabolism, cell extracts of
, before and after CdSe synthesis, were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that the amount of superoxide dismutase (SOD) decreased after CdSe synthesis. Fluorescence microscopy revealed that cytoplasmic superoxide increased significantly after CdSe synthesis. The accumulation of superoxide may increase the expression of various metabolites that play a role in reducing Se
to Se
and inhibit the aggregation of CdSe to make nanoparticles. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1996-1944 1996-1944 |
DOI: | 10.3390/ma9100855 |