Synthesis of CdSe Quantum Dots Using Fusarium oxysporum

• Published in: Materials Vol. 9; no. 10; p. 855
• Main Authors: Yamaguchi, Takaaki, Tsuruda, Yoshijiro, Furukawa, Tomohiro, Negishi, Lumi, Imura, Yuki, Sakuda, Shohei, Yoshimura, Etsuro, Suzuki, Michio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.10.2016; MDPI
• Subjects: Cadmium; Cadmium selenides; CdSe; Electrons; Fluorescence; Fusarium oxysporum; High temperature; Intermetallics; Medical research; Metabolites; Microorganisms; Nanoparticles; quantum dot; Quantum dots; Selenium; superoxide dismutase; Synthesis; Transmission electron microscopy; Japan; Germany; quantum dot; Fusarium oxysporum; CdSe; superoxide dismutase
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma9100855

CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using . The cadmium and selenium concentration, pH, and temperature for the culture of ( ) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial cells of were observed by transmission electron microscopy. Ultra-thin sections of showed that the CdSe quantum dots were precipitated in the intracellular space, indicating that cadmium and selenium ions were incorporated into the cell and that the quantum dots were synthesized with intracellular metabolites. To reveal differences in metabolism, cell extracts of , before and after CdSe synthesis, were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that the amount of superoxide dismutase (SOD) decreased after CdSe synthesis. Fluorescence microscopy revealed that cytoplasmic superoxide increased significantly after CdSe synthesis. The accumulation of superoxide may increase the expression of various metabolites that play a role in reducing Se to Se and inhibit the aggregation of CdSe to make nanoparticles.