Underweight rats have enhanced dopamine release and blunted acetylcholine response in the nucleus accumbens while bingeing on sucrose

• Published in: Neuroscience Vol. 156; no. 4; pp. 865 - 871
• Main Authors: Avena, N.M, Rada, P, Hoebel, B.G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 28.10.2008; Elsevier
• Subjects: Acetylcholine - metabolism; Analysis of Variance; Animals; Behavior, Animal; Biological and medical sciences; Body Weight - physiology; Bulimia; Chromatography, High Pressure Liquid - methods; Dopamine - metabolism; eating disorders; Food Deprivation - physiology; food restriction; Fundamental and applied biological sciences. Psychology; Male; Microdialysis; Neurology; Nucleus Accumbens - drug effects; Rats; Rats, Sprague-Dawley; Sucrose - administration & dosage; sugar; Sweetening Agents - administration & dosage; Time Factors; Vertebrates: nervous system and sense organs; DOPAC; 3,4-dihydroxy-phenylacetic acid; ACh; food restriction; homovanillic acid; eating disorders; nucleus accumbens; NAc; HVA; microdialysis; analysis of variance; ANOVA; high performance liquid chromatography with electrochemical detection; acetylcholine; sugar; DA; dopamine; HPLC-EC; Dopamine; Rat; Sucrose; Rodentia; Central nervous system; Basal ganglion; Catecholamine; Eating disorder; Feeding; Encephalon; Nucleus accumbens; Vertebrata; Mammalia; Microdialysis; Animal; Neurotransmitter; Acetylcholine; Restricted diet; Release
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2008.08.017

Abstract The present study tested whether rats release more accumbens dopamine (DA) during a sugar binge when they are underweight vs. normal weight. Since acetylcholine (ACh) in the nucleus accumbens (NAc) normally increases as a meal progresses and satiety ensues, we also tested whether ACh release is altered when an animal has lost weight. Rats were maintained on daily 8-h access to chow, with 10% sucrose solution available for the first 2 h. Microdialysis performed on day 21, at normal body weight, revealed an increase in extracellular DA to 122% of baseline in response to drinking sucrose. Extracellular ACh peaked at the end of the meal. Next, the rats were food and sucrose restricted so that by day 28 they were at 85% body weight. When retested, these animals released significantly more DA when drinking sucrose (179%), but ACh release failed to rise. A control group was tested in the same manner but given sugar only on days 1, 21 and 28. At normal body weight, control animals showed a non-significant rise in DA when drinking sucrose on day 21. On day 28, at 85% body weight, the controls showed a small increase (124%) in DA release; however, this was significantly lower than the 179% observed in the underweight rats with daily sugar access. These findings suggest that when an animal binges on sugar and then loses weight, the binge releases significantly more DA and less ACh than when animals are at a normal body weight.