Heat shock protein 90 is downregulated in calcific aortic valve disease

• Published in: BMC cardiovascular disorders Vol. 19; no. 1; p. 306
• Main Authors: Weisell, Jonna, Ohukainen, Pauli, Näpänkangas, Juha, Ohlmeier, Steffen, Bergmann, Ulrich, Peltonen, Tuomas, Taskinen, Panu, Ruskoaho, Heikki, Rysä, Jaana
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.12.2019; BioMed Central; BMC
• Subjects: Annexins; Anopheles; Aortic valve insufficiency; Aortic valve stenosis; Calcification (Physiology); Calcified aortic valve disease; Comparative analysis; EDTA; Electrophoresis; Health maintenance organizations; Heart valve diseases; Heat shock proteins; Heat-shock protein; Immunohistochemistry; Mass spectrometry; Novels; Proteomics; Spectroscopy; Stenosis; Surgery; Calcified aortic valve disease; Aortic valve stenosis; Heat-shock protein; Proteomics
• ISSN: 1471-2261; 1471-2261
• DOI: 10.1186/s12872-019-01294-2

Calcific aortic valve disease (CAVD) is an atheroinflammatory process; finally it leads to progressive calcification of the valve. There is no effective pharmacological treatment for CAVD and many of the underlying molecular mechanisms remain unknown. We conducted a proteomic study to reveal novel factors associated with CAVD. We compared aortic valves from patients undergoing valvular replacement surgery due to non-calcified aortic insufficiency (control group, n = 5) to a stenotic group (n = 7) using two-dimensional difference gel electrophoresis (2D-DIGE). Protein spots were identified with mass spectrometry. Western blot and immunohistochemistry were used to validate the results in a separate patient cohort and Ingenuity Pathway Analysis (IPA) was exploited to predict the regulatory network of CAVD. We detected an upregulation of complement 9 (C9), serum amyloid P-component (APCS) and transgelin as well as downregulation of heat shock protein (HSP90), protein disulfide isomerase A3 (PDIA3), annexin A2 (ANXA2) and galectin-1 in patients with aortic valve stenosis. The decreased protein expression of HSP90 was confirmed with Western blot. We describe here a novel data set of proteomic changes associated with CAVD, including downregulation of the pro-inflammatory cytosolic protein, HSP90.