Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth

Background Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment. Methods Her...

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Published inJournal of gastroenterology Vol. 47; no. 8; pp. 936 - 940
Main Authors Hirsch, Christian, Tegtmeyer, Nicole, Rohde, Manfred, Rowland, Marion, Oyarzabal, Omar A., Backert, Steffen
Format Journal Article
LanguageEnglish
Published Japan Springer Japan 01.08.2012
Springer
Springer Nature B.V
Subjects
Abdominal Surgery
Child, Preschool
Colorectal Surgery
Dental Caries - microbiology
Dental Plaque - microbiology
Dental Pulp Cavity - microbiology
Gastroenterology
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - isolation & purification
Hepatology
Humans
Male
Medicine
Medicine & Public Health
Microscopy, Electron, Scanning
Polymerase Chain Reaction
Rapid Communication
RNA
Root canal therapy
Surgical Oncology
Tooth, Deciduous - microbiology
Protein profiling
Deciduous teeth
Endodontics
Electron microscopy
Root canal
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Summary:Background Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment. Methods Here we used electron microscopy, selective growth techniques, urease assays, 16S rRNA PCR, and western blotting to investigate the possible presence of live H. pylori in 10 root canal and corresponding plaque samples of endodontic-infected deciduous teeth in three children. Results Although H. pylori DNA was verifiable by PCR in several plaque and root canal samples, bacterial colonies could only be grown from two root canals, but not from plaque. These colonies were unequivocally identified as H. pylori by microscopic, genetic, and biochemical approaches. Conclusions Our findings show that root canals of endodontic-infected teeth may be a reservoir for live H. pylori that could serve as a potential source for transmission.
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ISSN:0944-1174
1435-5922
DOI:10.1007/s00535-012-0618-8