A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples

• Published in: Global challenges Vol. 5; no. 4; pp. 2000068 - n/a
• Main Authors: Ramos‐Mandujano, Gerardo, Salunke, Rahul, Mfarrej, Sara, Rachmadi, Andri Taruna, Hala, Sharif, Xu, Jinna, Alofi, Fadwa S., Khogeer, Asim, Hashem, Anwar M., Almontashiri, Naif A. M., Alsomali, Afrah, Shinde, Digambar B., Hamdan, Samir, Hong, Pei‐Ying, Pain, Arnab, Li, Mo
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.04.2021; John Wiley and Sons Inc; Wiley
• Subjects: Acids; Automation; Biohazards; Chloroform; COVID-19; Deactivation; Influenza; Laboratories; magnetic nanoparticles; Nanoparticles; nucleic acid purification; Nucleic acids; Pandemics; Pathogens; Phenols; Public health; Reagents; Respiratory syncytial virus; Ribonucleic acid; RNA; SARS‐CoV‐2; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral diseases; Viruses; Wastewater; wastewater surveillance; Workflow; United States > US; influenza; nucleic acid purification; magnetic nanoparticles; SARS‐CoV‐2; wastewater surveillance

Molecular diagnosis and surveillance of pathogens such as SARS‐CoV‐2 depend on nucleic acid isolation. Pandemics at the scale of COVID‐19 can cause a global shortage of proprietary commercial reagents and BSL‐2 laboratories to safely perform testing. Therefore, alternative solutions are urgently nee...