Preparation, characterization, and evaluation of genipin crosslinked chitosan/gelatin three-dimensional scaffolds for liver tissue engineering applications
In liver tissue engineering, scaffolds with porous structure desgined to supply nutrient and oxygen exchange for three‐dimensional (3‐D) cells culture, and maintain liver functions. Meanwhile, genipin, as a natural crosslinker, is widely used to crosslink biomaterials in tissue engineering, with low...
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Published in | Journal of biomedical materials research. Part A Vol. 104; no. 8; pp. 1863 - 1870 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.08.2016
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | In liver tissue engineering, scaffolds with porous structure desgined to supply nutrient and oxygen exchange for three‐dimensional (3‐D) cells culture, and maintain liver functions. Meanwhile, genipin, as a natural crosslinker, is widely used to crosslink biomaterials in tissue engineering, with lower cytotoxicity and better biocompatibility. In present study, chitosan/gelatin 3‐D scaffolds crosslinked by genipin, glutaraldehyde or 1‐(3‐dimethylaminopropyl)−3‐ethyl‐carbodimide hydrochloride (EDC) were prepared and characterized by Fourier‐transform infrared (FT‐IR) and scanning electron microscopy (SEM). The biocompatibility of chitosan/gelatin scaffolds corsslinked with different crosslinkers was investigated by cell viability, morphology and liver specific functions. The result showed that the 1% and 2% genipin crosslinked chitosan/gelatin scaffolds possess ideal porosity. The genipin crosslinked 3‐D scaffolds possessed the best biocompatibility than that of the others, and maintained liver specific functions when HepG2 cells seeded on scaffolds. The cellular morphology of HepG2 cells seeded on scaffolds showed that cells could penetrate into the scaffolds and proliferate significantly. Therefore, genipin crosslinked chitosan/gelatin scaffolds could be a promising biomaterial used in liver tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1863–1870, 2016. |
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Bibliography: | ArticleID:JBMA35717 istex:F3C787FC19DC64A14A1D1BA034125AB73AC8C8DE ark:/67375/WNG-KD1H3WFK-G Postdoctoral Science Foundation of China - No. 2014M552639 National Natural Science Foundation of China - No. 81473542, 31500761 Specialized Research Fund for the Doctoral Program of Higher Education - No. 20131210110008 Conflict of interest: The authors declare that there are no conflicts of interest. These two authors contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1549-3296 1552-4965 |
DOI: | 10.1002/jbm.a.35717 |