Resveratrol affects in vitro rumen fermentation, methane production and prokaryotic community composition in a time‐ and diet‐specific manner

• Published in: Microbial biotechnology Vol. 13; no. 4; pp. 1118 - 1131
• Main Authors: Ma, Tao, Wu, W., Tu, Y., Zhang, N., Diao, Q.
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.07.2020; John Wiley and Sons Inc; Wiley
• Subjects: Abundance; Animal Feed - analysis; Animals; Biodegradation; Community composition; Diet; Digestion; Dry matter; Fatty acids; Fermentation; Flavonoids; Metabolites; Methane; Methane - metabolism; Microorganisms; Phylogenetics; Plant extracts; Polyphenols; Propionic acid; Quality control; Relative abundance; Resveratrol; Resveratrol - metabolism; Rumen
• ISSN: 1751-7915; 1751-7915
• DOI: 10.1111/1751-7915.13566

Summary This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high‐concentrate (HC) and high‐forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets (P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter (P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet (P = 0.045). The relative abundance of 10 bacterial genera was affected by the three‐way interaction of treatment, diet and time (P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales. Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition. Resveratrol inhibited in‐vitro methane production, which could be associated with the lower abundance of Methanobrevibacter. Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation.