五味子乙素对脑胶质瘤细胞U87增殖的影响及其机制探讨

目的：观察五味子乙素（ Sch B）对脑胶质瘤细胞U87增殖的影响,并探讨其可能机制。方法培养U87细胞,分为A、B、C、D组,D组加入含5%血清的培养液200μL,A、B、C组分别加入含50、100、200μg/mL Sch B的等量培养液,比较各组细胞增殖情况、细胞周期及细胞中Caspase-3、Bax、Bcl-2及Bax/Bcl-2。结果与D组比较, A、B、C组48 h和72 h的细胞OD值降低（P均＜0.05）。与A、D组比较,B组Caspase-3、Bax、Bax/Bcl-2的A值及阳性表达率高（P均＜0.05）。与D组比较,C组Caspase-3、Bax/Bcl-2的A值及阳性表...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 15; pp. 1 - 3
Main Author 吕红 苏子博 付仁 郑立影 潘正 齐玲 金宏
Format Magazine Article
LanguageChinese
Published 吉林医药学院临床医学院,吉林吉林,132013 2016
Subjects
Bax蛋白
Bcl-2蛋白
Caspase-3蛋白
五味子乙素
脑胶质瘤
脑胶质瘤
Bcl-2蛋白
Caspase-3 protein
Bax protein
Bcl-2 protein
glioma
五味子乙素
Bax蛋白
Schisandrin B
Caspase-3蛋白
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Summary:目的：观察五味子乙素（ Sch B）对脑胶质瘤细胞U87增殖的影响,并探讨其可能机制。方法培养U87细胞,分为A、B、C、D组,D组加入含5%血清的培养液200μL,A、B、C组分别加入含50、100、200μg/mL Sch B的等量培养液,比较各组细胞增殖情况、细胞周期及细胞中Caspase-3、Bax、Bcl-2及Bax/Bcl-2。结果与D组比较, A、B、C组48 h和72 h的细胞OD值降低（P均＜0.05）。与A、D组比较,B组Caspase-3、Bax、Bax/Bcl-2的A值及阳性表达率高（P均＜0.05）。与D组比较,C组Caspase-3、Bax/Bcl-2的A值及阳性表达率高（P均＜0.05）。与A组相比,C组Caspase-3的A值及阳性表达率高（P均＜0.05）。与D组比较,A组G1期、G2/M期细胞比例增加,P均＜0.05。结论 Sch B能明显抑制U87细胞增殖,诱导其凋亡,且呈一定的量效关系；其机制可能与上调细胞Caspase-3、Bax、Bcl-2表达有关。
Bibliography:Objective To investigate the effect of Schisandrin B （ Sch B） on the proliferation of glioma U87 cells and to explore its possible mechanism. Methods Glioma U87 cells were cultured and were divided into groups A, B, C and D. Group D was added with culture solution that containing 5% serum 200 μL, and groups A, B and C were added with the same amount of nutrient solution which respectively contained 50, 100 and 200μg/ml Sch B. The cell proliferation was compared between these groups. The cell cycle and the expression of Caspase-3, Bax, Bcl-2 and Bax/Bcl-2 were respec-tively detected by ELISA and immunohistochemistry. Results Compared with group D, the A value at 48 h and 72 h in the groups A, B and C was decreased. Compared with groups D and A, the A values of Caspase-3, Bax and Bax/Bcl-2 and the positive expression rate of group B were increased （all P〈0. 05）. Compared with group D, the A values of Caspase-3 and Bax/Bcl-2, and the positive expression of group C were increased （all P〈0. 05）. Compared with grou
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.15.001