Time-Dependent Analysis of Plasmalogens in the Hippocampus of an Alzheimer's Disease Mouse Model: A Role of Ethanolamine Plasmalogen

• Published in: Brain sciences Vol. 11; no. 12; p. 1603
• Main Authors: Azad, Abul Kalam, Sheikh, Abdullah Md, Haque, Md Ahsanul, Osago, Harumi, Sakai, Hiromichi, Shibly, Abu Zaffar, Yano, Shozo, Michikawa, Makoto, Hossain, Shahdat, Tabassum, Shatera, A, Garu, Zhou, Xiaojing, Zhang, Yuchi, Nagai, Atsushi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.12.2021; MDPI
• Subjects: Alzheimer's disease; Brain; Chromatography; Endosomes; Ethanolamine; Glial cells; Glycerone-phosphate O-acyltransferase; GNPAT; Hippocampus; LC-SRM/MS; Lipids; Localization; Mass spectrometry; Methods; Mutation; Neurodegeneration; Neurodegenerative diseases; Neuronal-glial interactions; Oxidative stress; Pathology; Peptides; Phagocytosis; plasmalogen; Proteins; Reactive oxygen species; ROS; Scientific imaging; Software; Solvents; Western blotting; United States > US; Japan; GNPAT; glial cells; phagocytosis; plasmalogen; LC-SRM/MS; ROS
• ISSN: 2076-3425; 2076-3425
• DOI: 10.3390/brainsci11121603

Plasmalogens are alkenyl-acyl glycerophospholipids and decreased in post-mortem Alzheimer's disease (AD) brains. The aim of this study is to investigate the time-dependent changes of plasmalogens in the hippocampus of an AD model mouse (J20). Plasmalogen levels at 3, 6, 9, 12 and 15 months were analyzed by liquid-chromatography-targeted-multiplexed-selected-reaction-monitoring-tandem-mass-spectrometry (LC-SRM/MS). Reactive oxygen species (ROS) levels were evaluated using dichlorofluorescein diacetate (DCF-DA). Plasmalogen synthesizing enzyme glycerone-phosphate O-acyltransferase (GNPAT) and late endosome marker Rab7 levels were quantified by Western blotting. GNPAT localization, changes of neuronal and glial cell numbers were evaluated by immunostaining. Compared to wild-type mice (WT), total plasmalogen-ethanolamine, but not plasmalogen-choline levels, were increased at 9 months and subsequently decreased at 15 months in J20 mice. A principal component analysis of plasmalogen-ethanolamine species could separate WT and J20 mice both at 9 and 15 months. Both GNPAT and Rab7 protein were increased in J20 mice at 9 months, whereas GNPAT was decreased at 15 months. ROS levels were increased in J20 mice except for 9 months. Our results suggest that increased plasmalogen-ethanolamine could counteract ROS levels and contribute to the phagocytosis process in J20 mice at 9 months. Such results might indicate a transient protective response of plasmalogen-ethanolamine in AD conditions.