Development of a genotyping method for potato [Solanum tuberosum] scab pathogens based on multiplex PCR

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 9; pp. 2324 - 2334
• Main Authors: Tagawa, M.(National Inst. of Advanced Industrial Science and Technology Tsukuba, Ibaraki (Japan)), Tamaki, H, Manome, A, Koyama, O, Kamagata, Y
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.09.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 16S rRNA gene; 16S-23S ITS; AGENT PATHOGENE; Animals; ARN; BACTERIOSE; BACTERIOSES; BACTERIOSIS; Biological and medical sciences; DNA Primers - genetics; DNA, Bacterial - analysis; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - genetics; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genetic Techniques; GENOTIPOS; GENOTYPE; GENOTYPES; multiplex PCR; ORGANISMOS PATOGENOS; PATHOGENS; PCR; Phylogeny; Plant Diseases - genetics; Polymerase Chain Reaction - methods; potato scab disease; RNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; RONA; SCABS; Sensitivity and Specificity; SOLANUM TUBEROSUM; Solanum tuberosum - genetics; STREPTOMYCES; Streptomyces - classification; Streptomyces - genetics; Streptomyces spp; TAVELURE; Streptomycetaceae; Disease; Solanum tuberosum; Streptomyces; Genotype; Method; potato scab disease; 16S rRNA gene; Actinomycetales; Polymerase chain reaction; multiplex PCR; Potato; 16S-23S ITS; Gene; Ribosomal RNA; Dicotyledones; Pathogenic; Angiospermae; Streptomyces spp; 16S-RNA; Bacteria; Actinomycetes; Spermatophyta; Solanaceae
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80234

Scab disease significantly damages potato and other root crops. Streptomyces scabiei, S. acidiscabiei, and S. turgidiscabiei are the best-known causal agents of this disease. We have developed a novel genotyping method for these potato scab pathogens using multiplex PCR, whose benefits include rapid and easy detection of multiple species. We designed a species-specific primer set (6 primers, 3 pairs) for the 16S rRNA genes and 16S-23S ITS regions of these potato scab pathogens. The specificity of the primer set was confirmed by testing 18 strains containing potato scab pathogens, other Streptomyces species, and strains of other genera. The application of the developed method to potato field soil and potato tissue samples resulted in the clear detection and identification of pathogens. Since this method is applicable to a large number of environmental samples, it is expected to be useful for a high-throughput analysis of soil and plant tissues of scab disease.