A case report of pulmonary Botrytis sp. infection in an apparently healthy individual

• Published in: BMC infectious diseases Vol. 19; no. 1; p. 684
• Main Authors: Hashimoto, Seishu, Tanaka, Eisaku, Ueyama, Masakuni, Terada, Satoru, Inao, Takashi, Kaji, Yusuke, Yasuda, Takehiro, Hajiro, Takashi, Nakagawa, Tatsuo, Noma, Satoshi, Honjo, Gen, Kobashi, Yoichiro, Abe, Noriyuki, Kamei, Katsuhiko, Taguchi, Yoshio
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02.08.2019; BioMed Central; BMC
• Subjects: Analysis; Asthma; Base sequence; Botrytis; Botrytis sp; Care and treatment; Case Report; CAT scans; Cotton trade; Diagnosis; Diagnostic imaging; DNA; DNA sequence analysis; DNA sequencing; Etiology (Medicine); Extrinsic allergic alveolitis; Fungi; Genes; Genetic aspects; Histochemistry; Host-bacteria relationships; Immunocompetent host; Lung diseases; Methenamine; Monosaccharides; Pathogenic microorganisms; Phosphates; Plant fungal diseases; Pneumonia; Pulmonary infection; RNA; Shock; Surgery; Tomography; Botrytis sp; DNA sequence analysis; Immunocompetent host; Pulmonary infection
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-019-4319-2

Botrytis species are well known fungal pathogens of various plants but have not been reported as human pathogens, except as allergenic precipitants of asthma and hypersensitivity pneumonitis. The asymptomatic patient was referred because of a nodule revealed by chest X-ray. Computed tomography (CT) showed a cavitary nodule in the right upper lobe of the lung. He underwent wedge resection of the nodule, which revealed necrotizing granulomas and a fungus ball containing Y-shaped filamentous fungi, which was confirmed histopathologically. Culture of the specimen yielded white to grayish cotton-like colonies with black sclerotia. We performed multilocus gene sequence analyses including three single-copy nuclear DNA genes encoding glyceraldehyde-3-phosphate dehydrogenase, heat-shock protein 60, and DNA-dependent RNA polymerase subunit II. The analyses revealed that the isolate was most similar to Botrytis elliptica. To date, the pulmonary Botrytis sp. infection has not recurred after lung resection and the patient did not require any additional medication. We report the first case of an immunocompetent patient with pulmonary Botrytis sp. infection, which has not recurred after lung resection without any additional medication. Precise evaluation is necessary for the diagnosis of pulmonary Botrytis infection because it is indistinguishable from other filamentous fungi both radiologically and by histopathology. The etiology and pathophysiology of pulmonary Botrytis infection remains unclear. Further accumulation and analysis of Botrytis cases is warranted.