A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

• Published in: Polish journal of microbiology Vol. 70; no. 4; pp. 479 - 487
• Main Authors: Ma, Zhongmei, Li, N A, Ning, Chengcheng, Liu, Yucheng, Guo, Yun, Ji, Chunhui, Zhu, Xiaozhen, Meng, Qingling, Xia, Xianzhu, Zhang, Xingxing, Cai, Xuepeng, Cai, Kuojun, Jun, Qiao
• Format: Journal Article
• Language: English
• Published: Poland Exeley Inc 01.12.2021; De Gruyter Poland; Sciendo
• Subjects: Acid resistance; Bacterial Proteins - metabolism; biofilm; Biofilms; Causes of; Chemical properties; Cloning; Complementation; Deoxyribonucleic acid; DNA; Electrophoresis; Electrophoretic mobility; Environmental stress; Food contamination; Food contamination & poisoning; Food poisoning; Food safety; Gene Expression Regulation, Bacterial; Genes; Genetic aspects; Genetic transcription; Laboratories; Microbial mats; Microbiological research; Microbiology; Oxidative stress; Parasitic diseases; Plasmids; Proteins; Regulatory mechanisms (biology); Salmonella; Salmonella Typhimurium; Salmonella typhimurium - genetics; STM0859 gene; Stress, Physiological; Tolerances; Transcription factors; Transcription Factors - genetics; China; United States > US; Japan; Germany; environmental stress; Salmonella Typhimurium; biofilm; STM0859 gene
• ISSN: 1733-1331; 2544-4646
• DOI: 10.33073/pjm-2021-045

subsp. serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the gene-deficient strain and the complementation strain were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST- strain were significantly weaker, as compared with those of Typhimurium SL1344 (ST-SL1344) wild strain ( < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, and , of ST- strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST- / . The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the gene, suggesting that STM0859 can promote the transcription of the gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.