Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean

• Published in: Theoretical and applied genetics Vol. 129; no. 2; pp. 445 - 451
• Main Authors: Ping, Jieqing, Fitzgerald, Joshua C., Zhang, Chunbao, Lin, Feng, Bai, Yonghe, Wang, Dechun, Aggarwal, Rajat, Rehman, Maqsood, Crasta, Oswald, Ma, Jianxin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2016; Springer; Springer Nature B.V
• Subjects: Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosomes; Cultivars; Disease resistance (Plants); Disease Resistance - genetics; Diseases and pests; DNA, Plant - genetics; Genes; Genes, Dominant; Genes, Plant; Genetic aspects; Genomes; Genotyping Techniques; Glycine max; Glycine max - genetics; Glycine max - microbiology; Health aspects; Inheritance Patterns; Life Sciences; Microsatellite Repeats; Observations; Original Article; Pathogens; Phytophthora; Phytophthora sojae; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant Proteins - genetics; Plant-pathogen relationships; Polymorphism, Single Nucleotide; Prevention; Single nucleotide polymorphisms; Soybeans; Water molds; United States > US; Bulk Segregant Analysis; Mapping Population; Plant Introduction; Simple Sequence Repeat Marker; Cetyl Trimethyl Ammonium Bromide
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-015-2638-2

KEY MESSAGE : Rps11 confers excellent resistance to predominant Phytophthora sojae isolates capable of defeating major Rps genes deployed into soybean production, representing a novel source of resistance for soybean cultivar enhancement. Phytophthora root and stem rot (PRSR), caused by the soil-borne pathogen Phytophthora sojae, is a devastating disease of soybean [Glycine max (L.) Merr.] throughout the world. Deploying resistant soybean cultivars is the most effective and environmentally friendly approach to managing this disease. The soybean landrace PI 594527 was found to carry excellent resistance to all P. sojae isolates examined, some of which were capable of overcoming the major Rps genesp, such as Rps1-k, Rps1-c, and Rps3-a, predominantly used for soybean protection in the past decades. A mapping population consisting of 58 F₂ individuals and 209 F₂:₃ families derived from a cross between PI 594527 and the susceptible cultivar ‘Williams’ was used to characterize the inheritance pattern of the resistance to P. soja (Rps) in PI 594527. It was found that the resistance was conferred by a single Rps gene, designated Rps11, which was initially defined as an ~5 Mb genomic region at the beginning of chromosome 7 by bulked segregant analysis (BSA) with a nucleotide polymorphism (SNP) chip comprising 7039 SNP markers. Subsequently, simple sequence repeat (SSR) markers in the defined region were used to genotype the F₂:₃ mapping population to map Rps11 to a 225.3 kb genomic region flanked by SSR markers BARCSOYSSR_07_0286 and BARCSOYSSR_07_0300, according to the soybean reference genome sequence. Particularly, an SSR marker (i.e., BARCSOYSSR_07_0295) was found to tightly co-segregate with Rps11 in the mapping population and can be effectively used for marker-assisted selection of this gene for development of resistant soybean cultivars.