Development and characterization of mutant winter wheat (Triticum aestivum L.) accessions resistant to the herbicide quizalofop

• Published in: Theoretical and applied genetics Vol. 128; no. 2; pp. 343 - 351
• Main Authors: Ostlie, Michael, Haley, Scott D, Anderson, Victoria, Shaner, Dale, Manmathan, Harish, Beil, Craig, Westra, Phillip
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.02.2015; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Agricultural research; Agriculture; alanine; Alleles; Alopecurus myosuroides; backcrossing; Biochemistry; Biomedical and Life Sciences; Biotechnology; Crops; DNA, Plant - genetics; Enzymes; ethyl methanesulfonate; Gene mutations; Genetic aspects; Genetic research; Genome, Plant; Genomes; Genotype; Health aspects; Herbicide resistance; Herbicide Resistance - genetics; Herbicides; Life Sciences; missense mutation; Mutagenesis; mutants; Mutation; Mutation, Missense; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant resistance; Propionates; Quinoxalines; quizalofop; Seeds; Sequence Analysis, DNA; Triticum - genetics; Triticum aestivum; valine; Wheat; Winter wheat; United States > US; Colorado; BC1F2 Progeny; Herbicide Resistance; Herbicide Dose; Mutant Accession; Wheat Accession
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-014-2434-4

KEY MESSAGE: New herbicide resistance traits in wheat were produced through the use of induced mutagenesis. While herbicide-resistant crops have become common in many agricultural systems, wheat has seen few introductions of herbicide resistance traits. A population of Hatcher winter wheat treated with ethyl methanesulfonate was screened with quizalofop to identify herbicide-resistant plants. Initial testing identified plants that survived multiple quizalofop applications. A series of experiments were designed to characterize this trait. In greenhouse studies the mutants exhibited high levels of quizalofop resistance compared to non-mutant wheat. Sequencing ACC1 revealed a novel missense mutation causing an alanine to valine change at position 2004 (Alopecurus myosuroides reference sequence). Plants carrying single mutations in wheat’s three genomes (A, B, D) were identified. Acetyl co-enzyme A carboxylase in resistant plants was 4- to 10-fold more tolerant to quizalofop. Populations of segregating backcross progenies were developed by crossing each of the three individual mutants with wild-type wheat. Experiments conducted with these populations confirmed largely normal segregation, with each mutant allele conferring an additive level of resistance. Further tests showed that the A genome mutation conferred the greatest resistance and the B genome mutation conferred the least resistance to quizalofop. The non-transgenic herbicide resistance trait identified will enhance weed control strategies in wheat.